Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21217 | 5' | -50.9 | NC_004778.3 | + | 40077 | 0.72 | 0.865864 |
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Target: 5'- aCGAGGugucguCGCAgugcGUCAaaucguGCACCGGCGc -3' miRNA: 3'- -GCUCCuu----GUGUaa--CAGU------UGUGGCCGC- -5' |
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| 21217 | 5' | -50.9 | NC_004778.3 | + | 14406 | 0.72 | 0.857957 |
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Target: 5'- gGGGGAGCGCGgacgcGUUAucACACCGGUc -3' miRNA: 3'- gCUCCUUGUGUaa---CAGU--UGUGGCCGc -5' |
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| 21217 | 5' | -50.9 | NC_004778.3 | + | 5200 | 0.72 | 0.855542 |
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Target: 5'- cCGAGGAACGCAUgcgagcaUGgcacguaauaggCGACACCaGCGa -3' miRNA: 3'- -GCUCCUUGUGUA-------ACa-----------GUUGUGGcCGC- -5' |
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| 21217 | 5' | -50.9 | NC_004778.3 | + | 120698 | 0.72 | 0.84149 |
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Target: 5'- aCGGGGuGGCGCGcca-CGGCGCCGGCGg -3' miRNA: 3'- -GCUCC-UUGUGUaacaGUUGUGGCCGC- -5' |
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| 21217 | 5' | -50.9 | NC_004778.3 | + | 93391 | 0.77 | 0.622739 |
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Target: 5'- cCGAGGAugucguGCACGgaGcCGACGCCGGUGg -3' miRNA: 3'- -GCUCCU------UGUGUaaCaGUUGUGGCCGC- -5' |
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| 21217 | 5' | -50.9 | NC_004778.3 | + | 84097 | 1.11 | 0.006337 |
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Target: 5'- gCGAGGAACACAUUGUCAACACCGGCGg -3' miRNA: 3'- -GCUCCUUGUGUAACAGUUGUGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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