Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21218 | 5' | -59.9 | NC_004778.3 | + | 53246 | 0.66 | 0.690251 |
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Target: 5'- cGGUUGCUgCGgcggcgguuGCUGCGGCGGCG-GUUg -3' miRNA: 3'- cUCAACGG-GC---------UGGCGCCGUCGCuCAA- -5' |
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| 21218 | 5' | -59.9 | NC_004778.3 | + | 43993 | 0.66 | 0.670004 |
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Target: 5'- ----cGCCCaACgCGCGGCcGGCGGGUa -3' miRNA: 3'- cucaaCGGGcUG-GCGCCG-UCGCUCAa -5' |
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| 21218 | 5' | -59.9 | NC_004778.3 | + | 19570 | 0.66 | 0.649635 |
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Target: 5'- -uGUcGCgCUGACCGCGGUAggcGUGAGUUu -3' miRNA: 3'- cuCAaCG-GGCUGGCGCCGU---CGCUCAA- -5' |
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| 21218 | 5' | -59.9 | NC_004778.3 | + | 38568 | 0.67 | 0.608795 |
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Target: 5'- uAGcgUGCCCGugCGCGGUcuGGUGAa-- -3' miRNA: 3'- cUCa-ACGGGCugGCGCCG--UCGCUcaa -5' |
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| 21218 | 5' | -59.9 | NC_004778.3 | + | 100473 | 0.67 | 0.598609 |
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Target: 5'- -cGUUGCCauguaCGGCCGCGGUuggacaggcguGGCGGGc- -3' miRNA: 3'- cuCAACGG-----GCUGGCGCCG-----------UCGCUCaa -5' |
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| 21218 | 5' | -59.9 | NC_004778.3 | + | 112634 | 0.67 | 0.578319 |
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Target: 5'- cGAGUU-CUCGGCCGCuGGC-GCGAGc- -3' miRNA: 3'- -CUCAAcGGGCUGGCG-CCGuCGCUCaa -5' |
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| 21218 | 5' | -59.9 | NC_004778.3 | + | 70273 | 0.68 | 0.517586 |
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Target: 5'- cGGcgUGCgCGGCCGCGGCauauuuaGGCGGGg- -3' miRNA: 3'- cUCa-ACGgGCUGGCGCCG-------UCGCUCaa -5' |
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| 21218 | 5' | -59.9 | NC_004778.3 | + | 103134 | 0.85 | 0.048239 |
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Target: 5'- cAGUUGCUCGugUGCGGCGGCGGGUg -3' miRNA: 3'- cUCAACGGGCugGCGCCGUCGCUCAa -5' |
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| 21218 | 5' | -59.9 | NC_004778.3 | + | 45619 | 1.05 | 0.001814 |
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Target: 5'- cGAGUUGCCCGACCGCGGCAGCGAGUUc -3' miRNA: 3'- -CUCAACGGGCUGGCGCCGUCGCUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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