Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21222 | 5' | -51.7 | NC_004778.3 | + | 108543 | 0.66 | 0.967194 |
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Target: 5'- aGCGUGGUCGGcGGCgUUuuguGCAaACGAUg -3' miRNA: 3'- -CGUACUAGCUcCCGgAAu---CGU-UGCUA- -5' |
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| 21222 | 5' | -51.7 | NC_004778.3 | + | 29348 | 0.67 | 0.952137 |
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Target: 5'- cGCGUGcgCGAuuGGCUgccgAGCGACGAc -3' miRNA: 3'- -CGUACuaGCUc-CCGGaa--UCGUUGCUa -5' |
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| 21222 | 5' | -51.7 | NC_004778.3 | + | 85451 | 0.68 | 0.938194 |
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Target: 5'- gGCAUGGaCGcGGGCgUUugcGGCGGCGAc -3' miRNA: 3'- -CGUACUaGCuCCCGgAA---UCGUUGCUa -5' |
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| 21222 | 5' | -51.7 | NC_004778.3 | + | 71155 | 0.68 | 0.927588 |
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Target: 5'- aGgGUGucggCGGGGGCgUUGGCGAgGAa -3' miRNA: 3'- -CgUACua--GCUCCCGgAAUCGUUgCUa -5' |
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| 21222 | 5' | -51.7 | NC_004778.3 | + | 48863 | 0.68 | 0.921888 |
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Target: 5'- cGCAguUGGUCGcgcacGGCCUUGGCGGCa-- -3' miRNA: 3'- -CGU--ACUAGCuc---CCGGAAUCGUUGcua -5' |
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| 21222 | 5' | -51.7 | NC_004778.3 | + | 25652 | 0.69 | 0.915923 |
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Target: 5'- aGCuUGcgCGGGGGCC--GGCGGCGu- -3' miRNA: 3'- -CGuACuaGCUCCCGGaaUCGUUGCua -5' |
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| 21222 | 5' | -51.7 | NC_004778.3 | + | 50914 | 1.09 | 0.00575 |
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Target: 5'- gGCAUGAUCGAGGGCCUUAGCAACGAUg -3' miRNA: 3'- -CGUACUAGCUCCCGGAAUCGUUGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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