Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21227 | 3' | -47.4 | NC_004778.3 | + | 89517 | 0.66 | 0.999203 |
Target: 5'- -uUCC--UUACaGUCUGGUCGAacCACAa -3' miRNA: 3'- uuAGGcaAAUGaCAGACCAGUU--GUGU- -5' |
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21227 | 3' | -47.4 | NC_004778.3 | + | 96911 | 0.67 | 0.997396 |
Target: 5'- cGUCgCGggUUAuCUGUCcacGGUCAACACAu -3' miRNA: 3'- uUAG-GCa-AAU-GACAGa--CCAGUUGUGU- -5' |
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21227 | 3' | -47.4 | NC_004778.3 | + | 31348 | 0.68 | 0.993008 |
Target: 5'- uGUCCGUgaa-UGUCggcGGUCAACAUc -3' miRNA: 3'- uUAGGCAaaugACAGa--CCAGUUGUGu -5' |
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21227 | 3' | -47.4 | NC_004778.3 | + | 59696 | 1.08 | 0.017722 |
Target: 5'- gAAUCCGUUUACUGUCUGGUCAACACAa -3' miRNA: 3'- -UUAGGCAAAUGACAGACCAGUUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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