miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21227 3' -47.4 NC_004778.3 + 89517 0.66 0.999203
Target:  5'- -uUCC--UUACaGUCUGGUCGAacCACAa -3'
miRNA:   3'- uuAGGcaAAUGaCAGACCAGUU--GUGU- -5'
21227 3' -47.4 NC_004778.3 + 96911 0.67 0.997396
Target:  5'- cGUCgCGggUUAuCUGUCcacGGUCAACACAu -3'
miRNA:   3'- uUAG-GCa-AAU-GACAGa--CCAGUUGUGU- -5'
21227 3' -47.4 NC_004778.3 + 31348 0.68 0.993008
Target:  5'- uGUCCGUgaa-UGUCggcGGUCAACAUc -3'
miRNA:   3'- uUAGGCAaaugACAGa--CCAGUUGUGu -5'
21227 3' -47.4 NC_004778.3 + 59696 1.08 0.017722
Target:  5'- gAAUCCGUUUACUGUCUGGUCAACACAa -3'
miRNA:   3'- -UUAGGCAAAUGACAGACCAGUUGUGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.