Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21234 | 3' | -49.7 | NC_004778.3 | + | 9701 | 0.66 | 0.995372 |
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Target: 5'- gGCCGGCGCGgCGUCguguuccaaaacGGCGCg -3' miRNA: 3'- -CGGCUGCGU-GCAGauuucua-----UCGUGa -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 115145 | 0.66 | 0.995444 |
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Target: 5'- cGCCaGCGCGgGUCaUAAuguGGUGGUGCUc -3' miRNA: 3'- -CGGcUGCGUgCAG-AUUu--CUAUCGUGA- -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 46692 | 0.66 | 0.995444 |
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Target: 5'- uGCaCGACGCACGUUUGgacaacaaaAAGAUcAGgACc -3' miRNA: 3'- -CG-GCUGCGUGCAGAU---------UUCUA-UCgUGa -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 69775 | 0.66 | 0.995444 |
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Target: 5'- aUCGAUGUAgCGcUCUGGAGGcgUGGCGCa -3' miRNA: 3'- cGGCUGCGU-GC-AGAUUUCU--AUCGUGa -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 21343 | 0.66 | 0.99612 |
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Target: 5'- -aCGGCuGCACGUCcgccGGcgAGCACUu -3' miRNA: 3'- cgGCUG-CGUGCAGauu-UCuaUCGUGA- -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 125005 | 0.66 | 0.99612 |
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Target: 5'- -aUGACGCAUGUCUGA----GGCAUUc -3' miRNA: 3'- cgGCUGCGUGCAGAUUucuaUCGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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