Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21234 | 3' | -49.7 | NC_004778.3 | + | 19884 | 1.06 | 0.015098 |
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Target: 5'- cGCUGACGCACGUCUAAAGAUAGCACUc -3' miRNA: 3'- -CGGCUGCGUGCAGAUUUCUAUCGUGA- -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 17928 | 0.7 | 0.954349 |
|
Target: 5'- cGCCGGCGUcaGCuUCUaAGAGAUGcGCGCa -3' miRNA: 3'- -CGGCUGCG--UGcAGA-UUUCUAU-CGUGa -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 14024 | 0.67 | 0.99049 |
|
Target: 5'- uGUCGAaGCGCa-CUGAAGGUAGUGCUu -3' miRNA: 3'- -CGGCUgCGUGcaGAUUUCUAUCGUGA- -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 9701 | 0.66 | 0.995372 |
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Target: 5'- gGCCGGCGCGgCGUCguguuccaaaacGGCGCg -3' miRNA: 3'- -CGGCUGCGU-GCAGauuucua-----UCGUGa -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 7167 | 0.69 | 0.971975 |
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Target: 5'- cGCCGA-GCACuagcUCguuGAGGUGGCGCa -3' miRNA: 3'- -CGGCUgCGUGc---AGau-UUCUAUCGUGa -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 4431 | 0.67 | 0.992823 |
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Target: 5'- gGCCGACGCAauuauuCG-CUucGAAGA-GGCGCg -3' miRNA: 3'- -CGGCUGCGU------GCaGA--UUUCUaUCGUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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