Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21234 | 3' | -49.7 | NC_004778.3 | + | 17928 | 0.7 | 0.954349 |
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Target: 5'- cGCCGGCGUcaGCuUCUaAGAGAUGcGCGCa -3' miRNA: 3'- -CGGCUGCG--UGcAGA-UUUCUAU-CGUGa -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 38610 | 0.68 | 0.979833 |
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Target: 5'- cGCCGGguuCGCGCGUUacAAGugcgAGCGCa -3' miRNA: 3'- -CGGCU---GCGUGCAGauUUCua--UCGUGa -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 62560 | 0.68 | 0.982047 |
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Target: 5'- uGCCGGCGUACGcgcaaaaguUCUAcaacGggGGCGCUa -3' miRNA: 3'- -CGGCUGCGUGC---------AGAUuu--CuaUCGUGA- -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 63334 | 0.68 | 0.984073 |
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Target: 5'- aGCCG-CGCACGUUUcAAAcGGU-GCACa -3' miRNA: 3'- -CGGCuGCGUGCAGA-UUU-CUAuCGUGa -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 31616 | 0.67 | 0.9876 |
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Target: 5'- aUCGGCggugguauuggGCGCGUCcAAAGAUcGCACUg -3' miRNA: 3'- cGGCUG-----------CGUGCAGaUUUCUAuCGUGA- -5' |
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| 21234 | 3' | -49.7 | NC_004778.3 | + | 125005 | 0.66 | 0.99612 |
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Target: 5'- -aUGACGCAUGUCUGA----GGCAUUc -3' miRNA: 3'- cgGCUGCGUGCAGAUUucuaUCGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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