Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21236 | 3' | -47.5 | NC_004778.3 | + | 95211 | 0.7 | 0.988118 |
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Target: 5'- gCCGGAaguagcgccGAUAGUACUCGUUgaggcCGGGg -3' miRNA: 3'- aGGUUU---------CUAUCGUGAGCGAaaa--GCCC- -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 122793 | 0.71 | 0.975476 |
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Target: 5'- -aCGuuuAUAGCACUCGCUUUUCGa- -3' miRNA: 3'- agGUuucUAUCGUGAGCGAAAAGCcc -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 122681 | 0.72 | 0.955038 |
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Target: 5'- gUCU----AUAGCACUCGCUUUUCGa- -3' miRNA: 3'- -AGGuuucUAUCGUGAGCGAAAAGCcc -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 122743 | 0.72 | 0.955038 |
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Target: 5'- gUCU----AUAGCACUCGCUUUUCGa- -3' miRNA: 3'- -AGGuuucUAUCGUGAGCGAAAAGCcc -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 19938 | 0.79 | 0.697996 |
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Target: 5'- gUUAAAGAUAGCACUUGUUUUUCGa- -3' miRNA: 3'- aGGUUUCUAUCGUGAGCGAAAAGCcc -5' |
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| 21236 | 3' | -47.5 | NC_004778.3 | + | 66879 | 1.12 | 0.010828 |
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Target: 5'- gUCCAAAGAUAGCACUCGCUUUUCGGGu -3' miRNA: 3'- -AGGUUUCUAUCGUGAGCGAAAAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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