Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21240 | 3' | -54 | NC_004778.3 | + | 100272 | 0.66 | 0.94207 |
Target: 5'- aGCuGUGCcUGUGAAAUAGuUGUCaCCGUc -3' miRNA: 3'- aUG-CACGcGCACUUUGUC-ACAG-GGCA- -5' |
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21240 | 3' | -54 | NC_004778.3 | + | 7484 | 0.66 | 0.92114 |
Target: 5'- -uUGUGCGC-UGGGAC-GUGUUCCGc -3' miRNA: 3'- auGCACGCGcACUUUGuCACAGGGCa -5' |
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21240 | 3' | -54 | NC_004778.3 | + | 33272 | 0.67 | 0.915274 |
Target: 5'- aGCG-GCGCGUGGAagaggucguugcACAGUcgGUCgCCGa -3' miRNA: 3'- aUGCaCGCGCACUU------------UGUCA--CAG-GGCa -5' |
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21240 | 3' | -54 | NC_004778.3 | + | 91335 | 0.67 | 0.909156 |
Target: 5'- -cCGUGCGCGUGAAcGCgAGcG-CCCGa -3' miRNA: 3'- auGCACGCGCACUU-UG-UCaCaGGGCa -5' |
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21240 | 3' | -54 | NC_004778.3 | + | 45712 | 0.69 | 0.817452 |
Target: 5'- cUACGUGCGCGUuaacGAcuuGCAGUGgaUUCCGc -3' miRNA: 3'- -AUGCACGCGCA----CUu--UGUCAC--AGGGCa -5' |
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21240 | 3' | -54 | NC_004778.3 | + | 37999 | 0.69 | 0.799316 |
Target: 5'- cGCGUGCGCGUcGAuaAGCAccacgcGUGUCgCCGc -3' miRNA: 3'- aUGCACGCGCA-CU--UUGU------CACAG-GGCa -5' |
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21240 | 3' | -54 | NC_004778.3 | + | 48941 | 0.71 | 0.710896 |
Target: 5'- gACGUGgGCGUGGgaGACGGUGgCgCCGa -3' miRNA: 3'- aUGCACgCGCACU--UUGUCACaG-GGCa -5' |
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21240 | 3' | -54 | NC_004778.3 | + | 80303 | 1.05 | 0.006171 |
Target: 5'- uUACGUGCGCGUGAAACAGUGUCCCGUc -3' miRNA: 3'- -AUGCACGCGCACUUUGUCACAGGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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