Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21241 | 3' | -58.5 | NC_004778.3 | + | 71055 | 0.66 | 0.728614 |
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Target: 5'- cGAGGCcgcguccaGCCGcUGCacagCGGCCugAUGUGa -3' miRNA: 3'- -UUCUG--------CGGCcACGa---GCCGGugUACAC- -5' |
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| 21241 | 3' | -58.5 | NC_004778.3 | + | 25656 | 0.67 | 0.708634 |
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Target: 5'- --uGCGCgGGgGC-CGGCgGCGUGUGg -3' miRNA: 3'- uucUGCGgCCaCGaGCCGgUGUACAC- -5' |
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| 21241 | 3' | -58.5 | NC_004778.3 | + | 105612 | 0.67 | 0.69854 |
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Target: 5'- cGAGGCGCgGGa--UCGGCCGCugcGUGa -3' miRNA: 3'- -UUCUGCGgCCacgAGCCGGUGua-CAC- -5' |
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| 21241 | 3' | -58.5 | NC_004778.3 | + | 63984 | 0.67 | 0.667958 |
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Target: 5'- --uACGCCGGaugUGUUgGGCCAUGUGUc -3' miRNA: 3'- uucUGCGGCC---ACGAgCCGGUGUACAc -5' |
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| 21241 | 3' | -58.5 | NC_004778.3 | + | 127936 | 0.67 | 0.657693 |
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Target: 5'- -cGACGCCucUGCUUGGuCCACGUGc- -3' miRNA: 3'- uuCUGCGGccACGAGCC-GGUGUACac -5' |
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| 21241 | 3' | -58.5 | NC_004778.3 | + | 59763 | 0.68 | 0.606223 |
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Target: 5'- cGGACGaCCGGUuuUCGGCCAa--GUGg -3' miRNA: 3'- uUCUGC-GGCCAcgAGCCGGUguaCAC- -5' |
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| 21241 | 3' | -58.5 | NC_004778.3 | + | 95639 | 0.69 | 0.552205 |
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Target: 5'- uAGcACGCCGGUGCcggccaacgaaauuUCGGUCACGUcgggGUGc -3' miRNA: 3'- uUC-UGCGGCCACG--------------AGCCGGUGUA----CAC- -5' |
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| 21241 | 3' | -58.5 | NC_004778.3 | + | 6797 | 0.7 | 0.525224 |
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Target: 5'- cAGAUGCgGGUGUUUGGUCACGUc-- -3' miRNA: 3'- uUCUGCGgCCACGAGCCGGUGUAcac -5' |
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| 21241 | 3' | -58.5 | NC_004778.3 | + | 30907 | 0.77 | 0.190432 |
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Target: 5'- cAAGAagUGCCGGUGCUgguuugCGGCCGCAUGaUGg -3' miRNA: 3'- -UUCU--GCGGCCACGA------GCCGGUGUAC-AC- -5' |
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| 21241 | 3' | -58.5 | NC_004778.3 | + | 80875 | 1.05 | 0.002324 |
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Target: 5'- cAAGACGCCGGUGCUCGGCCACAUGUGc -3' miRNA: 3'- -UUCUGCGGCCACGAGCCGGUGUACAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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