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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21242 | 3' | -48.4 | NC_004778.3 | + | 70648 | 0.66 | 0.997335 |
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Target: 5'- cGGCUACAUUGu--GCUCgacgaacAGCugCAg -3' miRNA: 3'- uUCGAUGUAACuguUGAGa------UCGugGU- -5' |
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| 21242 | 3' | -48.4 | NC_004778.3 | + | 21101 | 0.66 | 0.996817 |
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Target: 5'- aAGGUUACg--GGCAACUCgGGCgGCCGg -3' miRNA: 3'- -UUCGAUGuaaCUGUUGAGaUCG-UGGU- -5' |
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| 21242 | 3' | -48.4 | NC_004778.3 | + | 7355 | 0.66 | 0.996761 |
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Target: 5'- -uGCUGCGUUG-UAGCUUUAauaggcaacauguGCGCCAc -3' miRNA: 3'- uuCGAUGUAACuGUUGAGAU-------------CGUGGU- -5' |
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| 21242 | 3' | -48.4 | NC_004778.3 | + | 65765 | 0.66 | 0.996218 |
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Target: 5'- cGAGCcggGCggUGACGGCgaucauugGGCACCAg -3' miRNA: 3'- -UUCGa--UGuaACUGUUGaga-----UCGUGGU- -5' |
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| 21242 | 3' | -48.4 | NC_004778.3 | + | 64420 | 0.66 | 0.994743 |
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Target: 5'- -cGCUACAcgGAUc-CUCUGGCACgCAc -3' miRNA: 3'- uuCGAUGUaaCUGuuGAGAUCGUG-GU- -5' |
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| 21242 | 3' | -48.4 | NC_004778.3 | + | 94912 | 0.68 | 0.98561 |
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Target: 5'- aAGGCUACGUgagUGACuACgacAGCGCCAc -3' miRNA: 3'- -UUCGAUGUA---ACUGuUGagaUCGUGGU- -5' |
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| 21242 | 3' | -48.4 | NC_004778.3 | + | 82032 | 1.07 | 0.013383 |
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Target: 5'- aAAGCUACAUUGACAACUCUAGCACCAu -3' miRNA: 3'- -UUCGAUGUAACUGUUGAGAUCGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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