Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21245 | 3' | -55.2 | NC_004778.3 | + | 95353 | 0.68 | 0.802515 |
Target: 5'- gGCAGCAgC-CCGuCGGCAGCCggGCa-- -3' miRNA: 3'- -CGUUGUaGaGGC-GCCGUCGGa-UGcuu -5' |
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21245 | 3' | -55.2 | NC_004778.3 | + | 104135 | 0.66 | 0.898384 |
Target: 5'- uGCAGCGUCUCUuugGGCGGC--GCGAu -3' miRNA: 3'- -CGUUGUAGAGGcg-CCGUCGgaUGCUu -5' |
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21245 | 3' | -55.2 | NC_004778.3 | + | 114414 | 0.66 | 0.875138 |
Target: 5'- aCGACAUCUCCGC--CAGCCUugugccgcccgaauACGGu -3' miRNA: 3'- cGUUGUAGAGGCGccGUCGGA--------------UGCUu -5' |
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21245 | 3' | -55.2 | NC_004778.3 | + | 119620 | 0.66 | 0.898384 |
Target: 5'- cCGAgAUUUgcaCCGUGGC-GCCUACGGAc -3' miRNA: 3'- cGUUgUAGA---GGCGCCGuCGGAUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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