Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21246 | 3' | -54.4 | NC_004778.3 | + | 105291 | 0.66 | 0.92114 |
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Target: 5'- cGCGCCgcggcgCCcaaUUCUAUGuAGCCAUCGu- -3' miRNA: 3'- -CGCGGa-----GG---AGGGUACuUCGGUAGUug -5' |
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| 21246 | 3' | -54.4 | NC_004778.3 | + | 9067 | 0.66 | 0.915274 |
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Target: 5'- aGCGCCggCUCa-AUGAGuCCGUCGACg -3' miRNA: 3'- -CGCGGagGAGggUACUUcGGUAGUUG- -5' |
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| 21246 | 3' | -54.4 | NC_004778.3 | + | 36485 | 0.67 | 0.896173 |
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Target: 5'- cGCGCUuggCCUaCUAUGAAGCCgauccgGUUAACa -3' miRNA: 3'- -CGCGGa--GGAgGGUACUUCGG------UAGUUG- -5' |
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| 21246 | 3' | -54.4 | NC_004778.3 | + | 36151 | 0.68 | 0.843296 |
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Target: 5'- cCGCCgugcggugCCaUCCCAacgaguUGAcGGCCGUCGGCa -3' miRNA: 3'- cGCGGa-------GG-AGGGU------ACU-UCGGUAGUUG- -5' |
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| 21246 | 3' | -54.4 | NC_004778.3 | + | 61505 | 0.7 | 0.74137 |
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Target: 5'- gGCGCCgUCCgcaCAUGAAGCCGaaccugCGGCg -3' miRNA: 3'- -CGCGG-AGGaggGUACUUCGGUa-----GUUG- -5' |
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| 21246 | 3' | -54.4 | NC_004778.3 | + | 91066 | 1.13 | 0.001753 |
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Target: 5'- aGCGCCUCCUCCCAUGAAGCCAUCAACg -3' miRNA: 3'- -CGCGGAGGAGGGUACUUCGGUAGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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