Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21247 | 3' | -59.1 | NC_004778.3 | + | 70136 | 0.66 | 0.704027 |
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Target: 5'- gUUGCAgguuuuGCGGcgGGCGCGCGcaUUCGg -3' miRNA: 3'- -AACGU------CGCCuaCCGCGUGCagGAGCa -5' |
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| 21247 | 3' | -59.1 | NC_004778.3 | + | 1692 | 0.68 | 0.612001 |
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Target: 5'- -aGCAGCGcGAgcaGGCGCAUGcCCgacaugCGUa -3' miRNA: 3'- aaCGUCGC-CUa--CCGCGUGCaGGa-----GCA- -5' |
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| 21247 | 3' | -59.1 | NC_004778.3 | + | 71207 | 0.68 | 0.581307 |
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Target: 5'- gUGCAGCGGcUGGa-CGCGgCCUCGc -3' miRNA: 3'- aACGUCGCCuACCgcGUGCaGGAGCa -5' |
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| 21247 | 3' | -59.1 | NC_004778.3 | + | 89884 | 0.68 | 0.557988 |
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Target: 5'- gUGCAGCGuGGUGggcgcaagcggcuuGCGCGCGUUgUUGUa -3' miRNA: 3'- aACGUCGC-CUAC--------------CGCGUGCAGgAGCA- -5' |
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| 21247 | 3' | -59.1 | NC_004778.3 | + | 6652 | 0.71 | 0.43589 |
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Target: 5'- --aCAGCGGAUuggGGUGCACGcCCUCa- -3' miRNA: 3'- aacGUCGCCUA---CCGCGUGCaGGAGca -5' |
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| 21247 | 3' | -59.1 | NC_004778.3 | + | 113943 | 0.79 | 0.132001 |
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Target: 5'- -cGCGGCGGAUGGCGCACGaUgaCGUa -3' miRNA: 3'- aaCGUCGCCUACCGCGUGCaGgaGCA- -5' |
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| 21247 | 3' | -59.1 | NC_004778.3 | + | 94591 | 1.05 | 0.001912 |
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Target: 5'- uUUGCAGCGGAUGGCGCACGUCCUCGUg -3' miRNA: 3'- -AACGUCGCCUACCGCGUGCAGGAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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