Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21248 | 5' | -62.1 | NC_004778.3 | + | 89358 | 0.66 | 0.559729 |
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Target: 5'- ---aCGGCaa-UAGCCAGGCGCCCa- -3' miRNA: 3'- cagaGCCGgucGUCGGUCUGCGGGca -5' |
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| 21248 | 5' | -62.1 | NC_004778.3 | + | 7150 | 0.67 | 0.501729 |
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Target: 5'- cGUCUCGGCgGGCGGC---GCGCCg-- -3' miRNA: 3'- -CAGAGCCGgUCGUCGgucUGCGGgca -5' |
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| 21248 | 5' | -62.1 | NC_004778.3 | + | 112481 | 0.68 | 0.47372 |
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Target: 5'- -gCUCGcGCCAGCGGCCGauGAacucgaGCCgGUg -3' miRNA: 3'- caGAGC-CGGUCGUCGGU--CUg-----CGGgCA- -5' |
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| 21248 | 5' | -62.1 | NC_004778.3 | + | 32993 | 0.68 | 0.45549 |
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Target: 5'- aUgUCGGCCAcGUAgGCCAGccGCGUCCGa -3' miRNA: 3'- cAgAGCCGGU-CGU-CGGUC--UGCGGGCa -5' |
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| 21248 | 5' | -62.1 | NC_004778.3 | + | 1686 | 0.7 | 0.370518 |
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Target: 5'- ---gCGGCCAGCAGCgcgagCAGGCgcauGCCCGa -3' miRNA: 3'- cagaGCCGGUCGUCG-----GUCUG----CGGGCa -5' |
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| 21248 | 5' | -62.1 | NC_004778.3 | + | 90688 | 0.7 | 0.339694 |
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Target: 5'- uUCUCGGCguGCccAGCCAG-CGgCCGg -3' miRNA: 3'- cAGAGCCGguCG--UCGGUCuGCgGGCa -5' |
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| 21248 | 5' | -62.1 | NC_004778.3 | + | 21658 | 0.72 | 0.24678 |
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Target: 5'- gGUUUCGGCCGGCGGUgGcGGCGCCa-- -3' miRNA: 3'- -CAGAGCCGGUCGUCGgU-CUGCGGgca -5' |
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| 21248 | 5' | -62.1 | NC_004778.3 | + | 57076 | 0.73 | 0.21909 |
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Target: 5'- aUCUCGGCCAGUuugcGCCAgcagcgcggacGACGCuCCGUu -3' miRNA: 3'- cAGAGCCGGUCGu---CGGU-----------CUGCG-GGCA- -5' |
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| 21248 | 5' | -62.1 | NC_004778.3 | + | 94892 | 1.07 | 0.000877 |
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Target: 5'- gGUCUCGGCCAGCAGCCAGACGCCCGUg -3' miRNA: 3'- -CAGAGCCGGUCGUCGGUCUGCGGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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