Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21249 | 3' | -51.5 | NC_004778.3 | + | 21209 | 0.66 | 0.982681 |
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Target: 5'- aGCCGuugcuGGUGUCGCCGaaCUCGUGaacgugaaagccgUGCAg -3' miRNA: 3'- -UGGC-----UUACAGCGGC--GAGUACaa-----------ACGU- -5' |
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| 21249 | 3' | -51.5 | NC_004778.3 | + | 37977 | 0.66 | 0.979624 |
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Target: 5'- -aCGcGUGUCGCCGCgCGcUGUUUGa- -3' miRNA: 3'- ugGCuUACAGCGGCGaGU-ACAAACgu -5' |
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| 21249 | 3' | -51.5 | NC_004778.3 | + | 89863 | 0.66 | 0.977206 |
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Target: 5'- uGCCGcggaGAUGUUGCCGCUgugcagCGUGguggGCGc -3' miRNA: 3'- -UGGC----UUACAGCGGCGA------GUACaaa-CGU- -5' |
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| 21249 | 3' | -51.5 | NC_004778.3 | + | 12461 | 0.66 | 0.974584 |
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Target: 5'- aACCu--UGUCGCCGgUCAUGgccgagaugGCAa -3' miRNA: 3'- -UGGcuuACAGCGGCgAGUACaaa------CGU- -5' |
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| 21249 | 3' | -51.5 | NC_004778.3 | + | 88687 | 0.67 | 0.954122 |
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Target: 5'- gACgGGAUGUCGCCGCgCAgcaagcgGCGc -3' miRNA: 3'- -UGgCUUACAGCGGCGaGUacaaa--CGU- -5' |
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| 21249 | 3' | -51.5 | NC_004778.3 | + | 5047 | 0.69 | 0.92469 |
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Target: 5'- -aCGGAUGUCGCCGCgCGacUGgcgGCGu -3' miRNA: 3'- ugGCUUACAGCGGCGaGU--ACaaaCGU- -5' |
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| 21249 | 3' | -51.5 | NC_004778.3 | + | 115689 | 0.75 | 0.637661 |
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Target: 5'- --aGAuUGUCGCCGCUCAUGguacGCAc -3' miRNA: 3'- uggCUuACAGCGGCGAGUACaaa-CGU- -5' |
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| 21249 | 3' | -51.5 | NC_004778.3 | + | 83193 | 0.79 | 0.404515 |
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Target: 5'- uGCCGucUGUgCGCCGUUCAUGUUgUGCAa -3' miRNA: 3'- -UGGCuuACA-GCGGCGAGUACAA-ACGU- -5' |
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| 21249 | 3' | -51.5 | NC_004778.3 | + | 95029 | 1.09 | 0.005911 |
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Target: 5'- cACCGAAUGUCGCCGCUCAUGUUUGCAa -3' miRNA: 3'- -UGGCUUACAGCGGCGAGUACAAACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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