Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21256 | 3' | -56.4 | NC_004778.3 | + | 9895 | 0.67 | 0.810217 |
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Target: 5'- aGUG-CcGCC-CGGGCGCgGUGUGCGc -3' miRNA: 3'- gUACuGcCGGaGCUUGCGgCACACGU- -5' |
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| 21256 | 3' | -56.4 | NC_004778.3 | + | 9208 | 0.67 | 0.801291 |
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Target: 5'- -uUGAgcCGGCgCUCGAGCGCgGcgguuaacuUGUGCGa -3' miRNA: 3'- guACU--GCCG-GAGCUUGCGgC---------ACACGU- -5' |
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| 21256 | 3' | -56.4 | NC_004778.3 | + | 21136 | 0.67 | 0.799487 |
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Target: 5'- aCGUGccgcucuGCGGCgUCGGgaGCGCCGUgauuuugGUGCGu -3' miRNA: 3'- -GUAC-------UGCCGgAGCU--UGCGGCA-------CACGU- -5' |
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| 21256 | 3' | -56.4 | NC_004778.3 | + | 1711 | 0.67 | 0.79221 |
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Target: 5'- -uUGACGGCgCgcaUGAACauGCCGuUGUGCAu -3' miRNA: 3'- guACUGCCG-Ga--GCUUG--CGGC-ACACGU- -5' |
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| 21256 | 3' | -56.4 | NC_004778.3 | + | 81759 | 0.69 | 0.715056 |
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Target: 5'- gGUGGCGGaCCgacuGCGCCGguagGUGCGc -3' miRNA: 3'- gUACUGCC-GGagcuUGCGGCa---CACGU- -5' |
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| 21256 | 3' | -56.4 | NC_004778.3 | + | 32299 | 0.69 | 0.684677 |
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Target: 5'- aGUGgccGCGGCgcaUUgGAGCGCCGUcGUGCAg -3' miRNA: 3'- gUAC---UGCCG---GAgCUUGCGGCA-CACGU- -5' |
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| 21256 | 3' | -56.4 | NC_004778.3 | + | 35784 | 0.7 | 0.664189 |
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Target: 5'- -uUGGCGGUCUgCGAAUGgUGUGUGUu -3' miRNA: 3'- guACUGCCGGA-GCUUGCgGCACACGu -5' |
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| 21256 | 3' | -56.4 | NC_004778.3 | + | 85425 | 0.7 | 0.63328 |
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Target: 5'- --cGACGGCauggacgCGGGCGCCGUGUa-- -3' miRNA: 3'- guaCUGCCGga-----GCUUGCGGCACAcgu -5' |
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| 21256 | 3' | -56.4 | NC_004778.3 | + | 64927 | 0.74 | 0.40922 |
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Target: 5'- gCAUauUGGCCaUCG-GCGCCGUGUGCAc -3' miRNA: 3'- -GUAcuGCCGG-AGCuUGCGGCACACGU- -5' |
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| 21256 | 3' | -56.4 | NC_004778.3 | + | 102374 | 1.08 | 0.00255 |
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Target: 5'- uCAUGACGGCCUCGAACGCCGUGUGCAg -3' miRNA: 3'- -GUACUGCCGGAGCUUGCGGCACACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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