Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21257 | 3' | -54.7 | NC_004778.3 | + | 8745 | 0.66 | 0.897403 |
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Target: 5'- ---cGGCGccguGGCGCGCCACCccGUCc-- -3' miRNA: 3'- caaaCCGC----CCGUGUGGUGGu-CAGaau -5' |
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| 21257 | 3' | -54.7 | NC_004778.3 | + | 103407 | 0.66 | 0.890533 |
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Target: 5'- uGUUUGGCGGaCACgucguGCCGCCAuGUUg-- -3' miRNA: 3'- -CAAACCGCCcGUG-----UGGUGGU-CAGaau -5' |
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| 21257 | 3' | -54.7 | NC_004778.3 | + | 70123 | 0.66 | 0.890533 |
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Target: 5'- ---cGGCGGGCGCGCgCAuUCGGUa--- -3' miRNA: 3'- caaaCCGCCCGUGUG-GU-GGUCAgaau -5' |
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| 21257 | 3' | -54.7 | NC_004778.3 | + | 29380 | 0.67 | 0.868471 |
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Target: 5'- gGUUUGcCGGGCgACACCACCAcggcGUUg-- -3' miRNA: 3'- -CAAACcGCCCG-UGUGGUGGU----CAGaau -5' |
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| 21257 | 3' | -54.7 | NC_004778.3 | + | 18647 | 0.67 | 0.860652 |
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Target: 5'- ---aGGCGGGCGcCACC-CCAuUUUUAa -3' miRNA: 3'- caaaCCGCCCGU-GUGGuGGUcAGAAU- -5' |
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| 21257 | 3' | -54.7 | NC_004778.3 | + | 27248 | 0.67 | 0.860652 |
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Target: 5'- gGUUUGGCGGGauacgaGCGCCcaauUUAGUUUUGu -3' miRNA: 3'- -CAAACCGCCCg-----UGUGGu---GGUCAGAAU- -5' |
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| 21257 | 3' | -54.7 | NC_004778.3 | + | 14221 | 0.67 | 0.85261 |
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Target: 5'- -aUUGGUGGuGCAauacccuccUACCGCCAG-CUUGu -3' miRNA: 3'- caAACCGCC-CGU---------GUGGUGGUCaGAAU- -5' |
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| 21257 | 3' | -54.7 | NC_004778.3 | + | 97400 | 0.7 | 0.721374 |
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Target: 5'- --gUGGCGGcGCGCaaggaaGCCGCCGGuUCUa- -3' miRNA: 3'- caaACCGCC-CGUG------UGGUGGUC-AGAau -5' |
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| 21257 | 3' | -54.7 | NC_004778.3 | + | 121114 | 0.7 | 0.700656 |
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Target: 5'- ----aGCGGGCGCGgcccgcgccCCGCCAGUCa-- -3' miRNA: 3'- caaacCGCCCGUGU---------GGUGGUCAGaau -5' |
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| 21257 | 3' | -54.7 | NC_004778.3 | + | 103038 | 1.07 | 0.003423 |
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Target: 5'- aGUUUGGCGGGCACACCACCAGUCUUAg -3' miRNA: 3'- -CAAACCGCCCGUGUGGUGGUCAGAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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