Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21260 | 5' | -56.2 | NC_004778.3 | + | 71426 | 0.68 | 0.765242 |
Target: 5'- aGGCGA---GGGUGUC-GGCGGGGgCGu -3' miRNA: 3'- -UCGUUuuaUCCGCAGcCCGCUCCgGC- -5' |
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21260 | 5' | -56.2 | NC_004778.3 | + | 71450 | 0.68 | 0.765242 |
Target: 5'- aGGCGA---GGGUGUC-GGCGGGGgCGu -3' miRNA: 3'- -UCGUUuuaUCCGCAGcCCGCUCCgGC- -5' |
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21260 | 5' | -56.2 | NC_004778.3 | + | 71474 | 0.68 | 0.765242 |
Target: 5'- aGGCGA---GGGUGUC-GGCGGGGgCGu -3' miRNA: 3'- -UCGUUuuaUCCGCAGcCCGCUCCgGC- -5' |
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21260 | 5' | -56.2 | NC_004778.3 | + | 94734 | 0.67 | 0.820178 |
Target: 5'- cAGCAc---GGGCGUCuGGCugcuGGCCGa -3' miRNA: 3'- -UCGUuuuaUCCGCAGcCCGcu--CCGGC- -5' |
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21260 | 5' | -56.2 | NC_004778.3 | + | 121243 | 0.67 | 0.820178 |
Target: 5'- cGGCGAGuc-GGgGUCuGcGGCGGGGUCGc -3' miRNA: 3'- -UCGUUUuauCCgCAG-C-CCGCUCCGGC- -5' |
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21260 | 5' | -56.2 | NC_004778.3 | + | 71234 | 0.67 | 0.837184 |
Target: 5'- aGGCGA---GGGUGUC-GGCGAGGgUGu -3' miRNA: 3'- -UCGUUuuaUCCGCAGcCCGCUCCgGC- -5' |
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21260 | 5' | -56.2 | NC_004778.3 | + | 71282 | 0.67 | 0.837184 |
Target: 5'- cGGCGA---GGGUGUC-GGCGAGGgUGu -3' miRNA: 3'- -UCGUUuuaUCCGCAGcCCGCUCCgGC- -5' |
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21260 | 5' | -56.2 | NC_004778.3 | + | 114350 | 0.73 | 0.480722 |
Target: 5'- gAGCGGuuugacccuGcGCGccgCGGGCGAGGCCGg -3' miRNA: 3'- -UCGUUuuau-----C-CGCa--GCCCGCUCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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