Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21262 | 5' | -57.7 | NC_004778.3 | + | 112340 | 0.66 | 0.817783 |
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Target: 5'- -gGCGCCGaGaaCGaAGUgcaGUCGGCCGa -3' miRNA: 3'- uaCGUGGC-CgaGC-UCAag-UAGCCGGC- -5' |
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| 21262 | 5' | -57.7 | NC_004778.3 | + | 40019 | 0.66 | 0.800115 |
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Target: 5'- -gGCACguCGGCgagCGAGcacugcaaaUUCGUgGGCCGa -3' miRNA: 3'- uaCGUG--GCCGa--GCUC---------AAGUAgCCGGC- -5' |
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| 21262 | 5' | -57.7 | NC_004778.3 | + | 9067 | 0.66 | 0.772485 |
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Target: 5'- -aGCGCCGGCUCaauGAGUcCGUCGaCgGa -3' miRNA: 3'- uaCGUGGCCGAG---CUCAaGUAGCcGgC- -5' |
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| 21262 | 5' | -57.7 | NC_004778.3 | + | 27880 | 0.67 | 0.76301 |
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Target: 5'- uUGUACgCGGCggCGAGUguguggCGGCCa -3' miRNA: 3'- uACGUG-GCCGa-GCUCAagua--GCCGGc -5' |
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| 21262 | 5' | -57.7 | NC_004778.3 | + | 4856 | 0.67 | 0.743718 |
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Target: 5'- aAUGUuccccgauGCUGGCaCGAGUUCAcuccgugCGGCCGc -3' miRNA: 3'- -UACG--------UGGCCGaGCUCAAGUa------GCCGGC- -5' |
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| 21262 | 5' | -57.7 | NC_004778.3 | + | 93039 | 0.68 | 0.693928 |
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Target: 5'- -cGCugCGGCUcaagcuggacgcCGAgccGUUC-UCGGCCGa -3' miRNA: 3'- uaCGugGCCGA------------GCU---CAAGuAGCCGGC- -5' |
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| 21262 | 5' | -57.7 | NC_004778.3 | + | 112517 | 0.68 | 0.68276 |
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Target: 5'- uGUGCGCCgcguaacGGCUCGcGUUCGUaaGGUCGa -3' miRNA: 3'- -UACGUGG-------CCGAGCuCAAGUAg-CCGGC- -5' |
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| 21262 | 5' | -57.7 | NC_004778.3 | + | 8879 | 0.73 | 0.392163 |
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Target: 5'- -gGCGCCGGCggCGucaaAGUUC-UCGGCCa -3' miRNA: 3'- uaCGUGGCCGa-GC----UCAAGuAGCCGGc -5' |
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| 21262 | 5' | -57.7 | NC_004778.3 | + | 112646 | 0.97 | 0.010184 |
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Target: 5'- cAUGCACCGGCUCGAGUUC-UCGGCCGc -3' miRNA: 3'- -UACGUGGCCGAGCUCAAGuAGCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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