Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21263 | 5' | -50.6 | NC_004778.3 | + | 61889 | 0.66 | 0.980093 |
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Target: 5'- aACAAggUGUGGUGCGUauacaucGUGaGGCGCAAc -3' miRNA: 3'- aUGUU--GCAUUACGCG-------CACaUCGCGUU- -5' |
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| 21263 | 5' | -50.6 | NC_004778.3 | + | 17333 | 0.66 | 0.980325 |
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Target: 5'- uUACGugGgugUGGUGCGCGg--GGCGCu- -3' miRNA: 3'- -AUGUugC---AUUACGCGCacaUCGCGuu -5' |
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| 21263 | 5' | -50.6 | NC_004778.3 | + | 6833 | 0.66 | 0.980325 |
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Target: 5'- cACGACGg---GCGCccccuGUGUGGaCGCAGg -3' miRNA: 3'- aUGUUGCauuaCGCG-----CACAUC-GCGUU- -5' |
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| 21263 | 5' | -50.6 | NC_004778.3 | + | 43981 | 0.66 | 0.982537 |
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Target: 5'- gGCcGCGcAAUuaGCGCGcUGUAGCGCu- -3' miRNA: 3'- aUGuUGCaUUA--CGCGC-ACAUCGCGuu -5' |
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| 21263 | 5' | -50.6 | NC_004778.3 | + | 36511 | 0.66 | 0.982537 |
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Target: 5'- cUAUGACGUuggAGUGCaGCGUGccguuGCGCGAc -3' miRNA: 3'- -AUGUUGCA---UUACG-CGCACau---CGCGUU- -5' |
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| 21263 | 5' | -50.6 | NC_004778.3 | + | 2534 | 0.66 | 0.98586 |
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Target: 5'- gUGCAGCGUAuuaAUGCGgGcggcgaccuuuaguUGUcGCGCAAu -3' miRNA: 3'- -AUGUUGCAU---UACGCgC--------------ACAuCGCGUU- -5' |
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| 21263 | 5' | -50.6 | NC_004778.3 | + | 39112 | 0.66 | 0.986393 |
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Target: 5'- cGCAaguACGUGGUGCGCG-GcGGCGa-- -3' miRNA: 3'- aUGU---UGCAUUACGCGCaCaUCGCguu -5' |
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| 21263 | 5' | -50.6 | NC_004778.3 | + | 61793 | 0.66 | 0.986393 |
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Target: 5'- cGCGGCGUuggcGCGCGcUGgcuGCGCGg -3' miRNA: 3'- aUGUUGCAuua-CGCGC-ACau-CGCGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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