miRNA: 3'- -UAGcu-GUCuuUGCAGUGCGGGCGCCu -5'
miRNA: 3'- -UAGCUGUCUUUGCAGU-------GCGGgCGCCU- -5'
miRNA: 3'- -UAGCuGUCUUUGCa-GUGCGGGCGCCu -5'
miRNA: 3'- -UAGCUGUCuuUGCAGUg---------CGGGCGCCU- -5'
miRNA: 3'- -UAGCUGUCUUUGCAGUGCGGGCGCCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21273 | 5' | -55.6 | NC_004778.3 | + | 64146 | 0.71 | 0.623661 |
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Target: 5'- gGUCacuCAGc-GCGUCGCGCCCGUGGc -3' miRNA: 3'- -UAGcu-GUCuuUGCAGUGCGGGCGCCu -5' |
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| 21273 | 5' | -55.6 | NC_004778.3 | + | 71507 | 0.72 | 0.571128 |
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Target: 5'- aGUCGGCGGAGGCGUUGgccagggUGUCgGCGGAa -3' miRNA: 3'- -UAGCUGUCUUUGCAGU-------GCGGgCGCCU- -5' |
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| 21273 | 5' | -55.6 | NC_004778.3 | + | 94860 | 0.74 | 0.454285 |
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Target: 5'- gAUUG-CAGGAGCGcgCACGCUCGCGGu -3' miRNA: 3'- -UAGCuGUCUUUGCa-GUGCGGGCGCCu -5' |
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| 21273 | 5' | -55.6 | NC_004778.3 | + | 21080 | 0.78 | 0.278255 |
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Target: 5'- uGUCGACAG--GCGUCAgcgcggugcaGCCCGCGGAg -3' miRNA: 3'- -UAGCUGUCuuUGCAGUg---------CGGGCGCCU- -5' |
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| 21273 | 5' | -55.6 | NC_004778.3 | + | 2230 | 1.07 | 0.003814 |
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Target: 5'- gAUCGACAGAAACGUCACGCCCGCGGAc -3' miRNA: 3'- -UAGCUGUCUUUGCAGUGCGGGCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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