Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21276 | 3' | -54.8 | NC_004778.3 | + | 52063 | 0.66 | 0.927844 |
Target: 5'- uUCGGgcUUGGaGCUGUugUGUCCGUC-CGa -3' miRNA: 3'- -AGCU--AACCgCGACA--GCAGGCAGuGCa -5' |
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21276 | 3' | -54.8 | NC_004778.3 | + | 14694 | 0.67 | 0.89147 |
Target: 5'- ----aUGGaCGCgcgGUCGUCCGgCACGa -3' miRNA: 3'- agcuaACC-GCGa--CAGCAGGCaGUGCa -5' |
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21276 | 3' | -54.8 | NC_004778.3 | + | 33091 | 0.67 | 0.880329 |
Target: 5'- -----cGGCGgUGUCGUCCGcccaacacaguguguUCGCGUc -3' miRNA: 3'- agcuaaCCGCgACAGCAGGC---------------AGUGCA- -5' |
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21276 | 3' | -54.8 | NC_004778.3 | + | 125667 | 0.68 | 0.862543 |
Target: 5'- aCGGccUGGCGCUcGUUGUC-GUUACGUa -3' miRNA: 3'- aGCUa-ACCGCGA-CAGCAGgCAGUGCA- -5' |
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21276 | 3' | -54.8 | NC_004778.3 | + | 65437 | 0.68 | 0.846784 |
Target: 5'- aCG-UUGGCGCggugGgCGUCCaUCACGUc -3' miRNA: 3'- aGCuAACCGCGa---CaGCAGGcAGUGCA- -5' |
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21276 | 3' | -54.8 | NC_004778.3 | + | 95075 | 0.75 | 0.484525 |
Target: 5'- uUCGG-UGGCGCUGUCGUa-GUCACu- -3' miRNA: 3'- -AGCUaACCGCGACAGCAggCAGUGca -5' |
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21276 | 3' | -54.8 | NC_004778.3 | + | 7278 | 1.09 | 0.003638 |
Target: 5'- aUCGAUUGGCGCUGUCGUCCGUCACGUa -3' miRNA: 3'- -AGCUAACCGCGACAGCAGGCAGUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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