Results 21 - 31 of 31 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21284 | 3' | -46.6 | NC_004778.3 | + | 99474 | 0.71 | 0.986236 |
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Target: 5'- aACaCUCGUACUcGAAAAGCAGGgucGGCg -3' miRNA: 3'- -UGcGAGCAUGAcCUUUUUGUUCa--CUG- -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 112811 | 0.74 | 0.944603 |
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Target: 5'- uACGUUUGUACccGAAAAACAAgGUGACg -3' miRNA: 3'- -UGCGAGCAUGacCUUUUUGUU-CACUG- -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 94874 | 0.74 | 0.934356 |
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Target: 5'- gACGCcCGUGCUGGGAuuGCAGGa--- -3' miRNA: 3'- -UGCGaGCAUGACCUUuuUGUUCacug -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 23797 | 0.75 | 0.889658 |
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Target: 5'- aACGCUCGUACcaGAAAAGCGGGgucGGCg -3' miRNA: 3'- -UGCGAGCAUGacCUUUUUGUUCa--CUG- -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 23994 | 0.75 | 0.889658 |
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Target: 5'- aACGCUCGUACcaGAAAAGCGGGgucGGCg -3' miRNA: 3'- -UGCGAGCAUGacCUUUUUGUUCa--CUG- -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 24192 | 0.75 | 0.889658 |
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Target: 5'- aACGCUCGUACcaGAAAAGCGGGgucGGCg -3' miRNA: 3'- -UGCGAGCAUGacCUUUUUGUUCa--CUG- -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 99350 | 0.78 | 0.794174 |
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Target: 5'- aACGCUCGUGCccGAAAAGCGAGgucGGCg -3' miRNA: 3'- -UGCGAGCAUGacCUUUUUGUUCa--CUG- -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 19912 | 0.78 | 0.794174 |
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Target: 5'- aACGCUCGUGCccGAAAAGCGAGgucGGCg -3' miRNA: 3'- -UGCGAGCAUGacCUUUUUGUUCa--CUG- -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 66721 | 0.78 | 0.784256 |
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Target: 5'- gACGCUCGUGCccGAAAAGCGAGgucGGCg -3' miRNA: 3'- -UGCGAGCAUGacCUUUUUGUUCa--CUG- -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 66590 | 0.84 | 0.482087 |
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Target: 5'- aACGCUCGUACUcGAAAAGCGAGgucGGCg -3' miRNA: 3'- -UGCGAGCAUGAcCUUUUUGUUCa--CUG- -5' |
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| 21284 | 3' | -46.6 | NC_004778.3 | + | 19940 | 1.02 | 0.053691 |
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Target: 5'- aACGCUCGUACUGGAAAAACA-GUGACg -3' miRNA: 3'- -UGCGAGCAUGACCUUUUUGUuCACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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