miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21293 3' -52 NC_004778.3 + 29414 0.66 0.965933
Target:  5'- -aUCGCGGA---GGCAGGCauGUUUCAc -3'
miRNA:   3'- ucAGCGUCUuuuUCGUCCGc-CAGAGU- -5'
21293 3' -52 NC_004778.3 + 12676 0.67 0.958734
Target:  5'- gGGagGCGGGAuAGGUAuGGCGGUUUUc -3'
miRNA:   3'- -UCagCGUCUUuUUCGU-CCGCCAGAGu -5'
21293 3' -52 NC_004778.3 + 2633 0.68 0.93004
Target:  5'- uGUCGCcaaacacguGAAGCGGGUGGUCg-- -3'
miRNA:   3'- uCAGCGucuu-----UUUCGUCCGCCAGagu -5'
21293 3' -52 NC_004778.3 + 98922 0.69 0.900934
Target:  5'- cGUCGCuGggGAuGguGGCGG-CUUg -3'
miRNA:   3'- uCAGCGuCuuUUuCguCCGCCaGAGu -5'
21293 3' -52 NC_004778.3 + 10018 0.69 0.900934
Target:  5'- cGG-CGCGuAAGGAGCcaauauuuGGGCGGUCUCc -3'
miRNA:   3'- -UCaGCGUcUUUUUCG--------UCCGCCAGAGu -5'
21293 3' -52 NC_004778.3 + 29221 1.09 0.005157
Target:  5'- cAGUCGCAGAAAAAGCAGGCGGUCUCAu -3'
miRNA:   3'- -UCAGCGUCUUUUUCGUCCGCCAGAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.