Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21293 | 3' | -52 | NC_004778.3 | + | 29414 | 0.66 | 0.965933 |
Target: 5'- -aUCGCGGA---GGCAGGCauGUUUCAc -3' miRNA: 3'- ucAGCGUCUuuuUCGUCCGc-CAGAGU- -5' |
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21293 | 3' | -52 | NC_004778.3 | + | 12676 | 0.67 | 0.958734 |
Target: 5'- gGGagGCGGGAuAGGUAuGGCGGUUUUc -3' miRNA: 3'- -UCagCGUCUUuUUCGU-CCGCCAGAGu -5' |
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21293 | 3' | -52 | NC_004778.3 | + | 2633 | 0.68 | 0.93004 |
Target: 5'- uGUCGCcaaacacguGAAGCGGGUGGUCg-- -3' miRNA: 3'- uCAGCGucuu-----UUUCGUCCGCCAGagu -5' |
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21293 | 3' | -52 | NC_004778.3 | + | 98922 | 0.69 | 0.900934 |
Target: 5'- cGUCGCuGggGAuGguGGCGG-CUUg -3' miRNA: 3'- uCAGCGuCuuUUuCguCCGCCaGAGu -5' |
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21293 | 3' | -52 | NC_004778.3 | + | 10018 | 0.69 | 0.900934 |
Target: 5'- cGG-CGCGuAAGGAGCcaauauuuGGGCGGUCUCc -3' miRNA: 3'- -UCaGCGUcUUUUUCG--------UCCGCCAGAGu -5' |
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21293 | 3' | -52 | NC_004778.3 | + | 29221 | 1.09 | 0.005157 |
Target: 5'- cAGUCGCAGAAAAAGCAGGCGGUCUCAu -3' miRNA: 3'- -UCAGCGUCUUUUUCGUCCGCCAGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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