miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21299 3' -53.6 NC_004778.3 + 114825 0.66 0.959769
Target:  5'- gGGCCuaCGUgACUaaGUGGggccgugcgcaaacGCUUCGCGg -3'
miRNA:   3'- -CCGGcgGCA-UGAaaCACC--------------UGAAGCGC- -5'
21299 3' -53.6 NC_004778.3 + 121378 0.66 0.95715
Target:  5'- -cUCGCCGcAgUUUGUGGACguuaGCGa -3'
miRNA:   3'- ccGGCGGCaUgAAACACCUGaag-CGC- -5'
21299 3' -53.6 NC_004778.3 + 108308 0.66 0.95715
Target:  5'- aGGCUGCCG-AgUUUGUaGcUUUCGCGa -3'
miRNA:   3'- -CCGGCGGCaUgAAACAcCuGAAGCGC- -5'
21299 3' -53.6 NC_004778.3 + 34682 0.66 0.949032
Target:  5'- cGCCGCCGcACUUgaUGGACauaagcagcaCGCGg -3'
miRNA:   3'- cCGGCGGCaUGAAacACCUGaa--------GCGC- -5'
21299 3' -53.6 NC_004778.3 + 64169 0.67 0.939953
Target:  5'- uGGCCGCCGaGCUUgacgcguucGUGGugacgaACgaCGCGg -3'
miRNA:   3'- -CCGGCGGCaUGAAa--------CACC------UGaaGCGC- -5'
21299 3' -53.6 NC_004778.3 + 39519 0.68 0.915333
Target:  5'- uGCUGCCcgacaacauggugcaGUACUUUGUGGGCacggUGCu -3'
miRNA:   3'- cCGGCGG---------------CAUGAAACACCUGaa--GCGc -5'
21299 3' -53.6 NC_004778.3 + 33609 0.68 0.912945
Target:  5'- cGCCGCCGUGC-----GGACggcCGCGc -3'
miRNA:   3'- cCGGCGGCAUGaaacaCCUGaa-GCGC- -5'
21299 3' -53.6 NC_004778.3 + 67636 0.69 0.85719
Target:  5'- cGGCaCG-UGUACgagGUGGACUUCGaCGc -3'
miRNA:   3'- -CCG-GCgGCAUGaaaCACCUGAAGC-GC- -5'
21299 3' -53.6 NC_004778.3 + 57017 0.69 0.841032
Target:  5'- uGCCGCCuacACUUUGUGGcgcagcccGCUaugUCGCGu -3'
miRNA:   3'- cCGGCGGca-UGAAACACC--------UGA---AGCGC- -5'
21299 3' -53.6 NC_004778.3 + 89877 0.72 0.729943
Target:  5'- uGCCGCUGUGCagcgugGUGGGCgcaaGCGg -3'
miRNA:   3'- cCGGCGGCAUGaaa---CACCUGaag-CGC- -5'
21299 3' -53.6 NC_004778.3 + 71215 0.75 0.534334
Target:  5'- aGGCCGCUGUGCagcgGcUGGACgcggccUCGCGa -3'
miRNA:   3'- -CCGGCGGCAUGaaa-C-ACCUGa-----AGCGC- -5'
21299 3' -53.6 NC_004778.3 + 33187 1.1 0.003714
Target:  5'- gGGCCGCCGUACUUUGUGGACUUCGCGc -3'
miRNA:   3'- -CCGGCGGCAUGAAACACCUGAAGCGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.