Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21300 | 5' | -51.9 | NC_004778.3 | + | 14559 | 0.66 | 0.974778 |
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Target: 5'- cGUCCAUGUgc--GCcagCAUUGUGGACGg -3' miRNA: 3'- -CAGGUACGaguaCGa--GUAGCGCUUGC- -5' |
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| 21300 | 5' | -51.9 | NC_004778.3 | + | 95997 | 0.66 | 0.974778 |
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Target: 5'- -cCCGUGCUUAcgGCgcgCAUCGUGuuGCGc -3' miRNA: 3'- caGGUACGAGUa-CGa--GUAGCGCu-UGC- -5' |
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| 21300 | 5' | -51.9 | NC_004778.3 | + | 16159 | 0.67 | 0.962222 |
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Target: 5'- -gCUGUGCgaCGUGCgCGUCGCGGAUa -3' miRNA: 3'- caGGUACGa-GUACGaGUAGCGCUUGc -5' |
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| 21300 | 5' | -51.9 | NC_004778.3 | + | 23421 | 0.67 | 0.958504 |
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Target: 5'- cGUCUGggucGCUCAUG-UCGUgCGCGGACa -3' miRNA: 3'- -CAGGUa---CGAGUACgAGUA-GCGCUUGc -5' |
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| 21300 | 5' | -51.9 | NC_004778.3 | + | 63954 | 0.67 | 0.958504 |
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Target: 5'- uGUCaaaGUGCgCGUGUUUuUCGCGGACu -3' miRNA: 3'- -CAGg--UACGaGUACGAGuAGCGCUUGc -5' |
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| 21300 | 5' | -51.9 | NC_004778.3 | + | 11634 | 0.67 | 0.954133 |
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Target: 5'- gGUCCAcgauuaauuuuUGUUCGguaUGCUCAUCGacaccgcCGGACGa -3' miRNA: 3'- -CAGGU-----------ACGAGU---ACGAGUAGC-------GCUUGC- -5' |
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| 21300 | 5' | -51.9 | NC_004778.3 | + | 38012 | 0.7 | 0.872223 |
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Target: 5'- cGUCCuUGCUUAaagugcacGCUCG-CGCGAACGu -3' miRNA: 3'- -CAGGuACGAGUa-------CGAGUaGCGCUUGC- -5' |
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| 21300 | 5' | -51.9 | NC_004778.3 | + | 98279 | 0.7 | 0.856476 |
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Target: 5'- uUCCAUGCUCAauUGCgUCAgCGCcAGCGc -3' miRNA: 3'- cAGGUACGAGU--ACG-AGUaGCGcUUGC- -5' |
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| 21300 | 5' | -51.9 | NC_004778.3 | + | 33514 | 1.1 | 0.005144 |
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Target: 5'- uGUCCAUGCUCAUGCUCAUCGCGAACGa -3' miRNA: 3'- -CAGGUACGAGUACGAGUAGCGCUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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