Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21304 | 5' | -61.8 | NC_004778.3 | + | 56773 | 0.69 | 0.425519 |
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Target: 5'- cGGUGCUgGUCGUGUU---UCUGCCCc -3' miRNA: 3'- aCCGCGGgCGGCACAGcauGGACGGG- -5' |
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| 21304 | 5' | -61.8 | NC_004778.3 | + | 90692 | 0.69 | 0.469615 |
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Target: 5'- cGGCguGCCCagccagcgGCCGguacgaaUCGUACCUGgCCCa -3' miRNA: 3'- aCCG--CGGG--------CGGCac-----AGCAUGGAC-GGG- -5' |
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| 21304 | 5' | -61.8 | NC_004778.3 | + | 85274 | 0.68 | 0.486976 |
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Target: 5'- cGGCGCCCG-CGUccaugccGUCGccGCCgcaaacGCCCg -3' miRNA: 3'- aCCGCGGGCgGCA-------CAGCa-UGGa-----CGGG- -5' |
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| 21304 | 5' | -61.8 | NC_004778.3 | + | 83541 | 0.67 | 0.544586 |
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Target: 5'- aUGGCuCgUCGCgCGUGUCGUugU-GCCCg -3' miRNA: 3'- -ACCGcG-GGCG-GCACAGCAugGaCGGG- -5' |
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| 21304 | 5' | -61.8 | NC_004778.3 | + | 51766 | 0.66 | 0.603243 |
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Target: 5'- uUGGuUGUagUGCCGguagGUCGUACC-GCCCc -3' miRNA: 3'- -ACC-GCGg-GCGGCa---CAGCAUGGaCGGG- -5' |
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| 21304 | 5' | -61.8 | NC_004778.3 | + | 32284 | 0.66 | 0.603243 |
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Target: 5'- cGGCGgC-GCCGUGUCGgGCUggUGCUUa -3' miRNA: 3'- aCCGCgGgCGGCACAGCaUGG--ACGGG- -5' |
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| 21304 | 5' | -61.8 | NC_004778.3 | + | 14727 | 0.66 | 0.632925 |
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Target: 5'- aGGUGCCCGgU---UCGUACCcgcUGUCCg -3' miRNA: 3'- aCCGCGGGCgGcacAGCAUGG---ACGGG- -5' |
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| 21304 | 5' | -61.8 | NC_004778.3 | + | 4556 | 0.66 | 0.642827 |
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Target: 5'- cGGCGCCCagcgcGCUGUGccgCGUuagcagacugGCCacUGUCCa -3' miRNA: 3'- aCCGCGGG-----CGGCACa--GCA----------UGG--ACGGG- -5' |
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| 21304 | 5' | -61.8 | NC_004778.3 | + | 32456 | 0.66 | 0.642827 |
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Target: 5'- cGGCGCagCCGCugCGUGUCGccUUUGCaCCa -3' miRNA: 3'- aCCGCG--GGCG--GCACAGCauGGACG-GG- -5' |
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| 21304 | 5' | -61.8 | NC_004778.3 | + | 35213 | 1.11 | 0.000594 |
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Target: 5'- uUGGCGCCCGCCGUGUCGUACCUGCCCu -3' miRNA: 3'- -ACCGCGGGCGGCACAGCAUGGACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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