Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21305 | 3' | -50 | NC_004778.3 | + | 7894 | 0.66 | 0.993391 |
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Target: 5'- gUGGAcGCGCCGAAaaguuugcGCGaGcauugGUUCGGCAu -3' miRNA: 3'- aACCU-UGUGGCUU--------UGC-Ca----CAAGCUGU- -5' |
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| 21305 | 3' | -50 | NC_004778.3 | + | 94969 | 0.66 | 0.992356 |
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Target: 5'- -cGGu---CCGAGACGcGcUGUUCGACGa -3' miRNA: 3'- aaCCuuguGGCUUUGC-C-ACAAGCUGU- -5' |
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| 21305 | 3' | -50 | NC_004778.3 | + | 84178 | 0.68 | 0.978759 |
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Target: 5'- -aGGAugGCUGGGacauuuuucuGCGGcccaUGUUCGGCAu -3' miRNA: 3'- aaCCUugUGGCUU----------UGCC----ACAAGCUGU- -5' |
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| 21305 | 3' | -50 | NC_004778.3 | + | 94753 | 0.68 | 0.97625 |
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Target: 5'- cUGGccGAgACCGAAACGGgGUUCG-CGc -3' miRNA: 3'- aACC--UUgUGGCUUUGCCaCAAGCuGU- -5' |
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| 21305 | 3' | -50 | NC_004778.3 | + | 2201 | 0.68 | 0.97625 |
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Target: 5'- -aGGAACGCgGcuGCGGUGgcggcCGACu -3' miRNA: 3'- aaCCUUGUGgCuuUGCCACaa---GCUGu -5' |
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| 21305 | 3' | -50 | NC_004778.3 | + | 39704 | 0.69 | 0.960387 |
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Target: 5'- uUUGG-GCACCGGAAUGGUGUagaACAa -3' miRNA: 3'- -AACCuUGUGGCUUUGCCACAagcUGU- -5' |
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| 21305 | 3' | -50 | NC_004778.3 | + | 35631 | 1.07 | 0.011995 |
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Target: 5'- cUUGGAACACCGAAACGGUGUUCGACAa -3' miRNA: 3'- -AACCUUGUGGCUUUGCCACAAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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