Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21312 | 5' | -61.5 | NC_004778.3 | + | 82258 | 0.67 | 0.558293 |
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Target: 5'- cUCCUCCAGCGU--GGUGCGUUUgUCg- -3' miRNA: 3'- -AGGAGGUCGCGgaCCGCGCGGA-AGac -5' |
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| 21312 | 5' | -61.5 | NC_004778.3 | + | 34870 | 0.67 | 0.558293 |
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Target: 5'- gCUUCUuGU-CUUGGCGCGCCUUCa- -3' miRNA: 3'- aGGAGGuCGcGGACCGCGCGGAAGac -5' |
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| 21312 | 5' | -61.5 | NC_004778.3 | + | 43130 | 0.67 | 0.538644 |
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Target: 5'- gCUUCgaCGGCGCCgcGGCGCGCCg---- -3' miRNA: 3'- aGGAG--GUCGCGGa-CCGCGCGGaagac -5' |
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| 21312 | 5' | -61.5 | NC_004778.3 | + | 119017 | 0.67 | 0.528908 |
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Target: 5'- gUCCU-CAGuUGCgUGcGCGCGCCgugUCUGu -3' miRNA: 3'- -AGGAgGUC-GCGgAC-CGCGCGGa--AGAC- -5' |
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| 21312 | 5' | -61.5 | NC_004778.3 | + | 77342 | 0.68 | 0.499166 |
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Target: 5'- gUCCUCCucGuCGUCcuccuugUGGCGCGCCgcgcgCUGg -3' miRNA: 3'- -AGGAGGu-C-GCGG-------ACCGCGCGGaa---GAC- -5' |
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| 21312 | 5' | -61.5 | NC_004778.3 | + | 32125 | 0.68 | 0.461938 |
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Target: 5'- gUCCUUCAGCGCCUgcacgacGGCGCuCCa---- -3' miRNA: 3'- -AGGAGGUCGCGGA-------CCGCGcGGaagac -5' |
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| 21312 | 5' | -61.5 | NC_004778.3 | + | 70368 | 0.69 | 0.418417 |
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Target: 5'- cUgUCCGGCGCUUGG-GCGCCguagauaauuUUCUGc -3' miRNA: 3'- aGgAGGUCGCGGACCgCGCGG----------AAGAC- -5' |
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| 21312 | 5' | -61.5 | NC_004778.3 | + | 8740 | 0.77 | 0.142655 |
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Target: 5'- gCCgCCGGCGCCgUGGCGCGCCa---- -3' miRNA: 3'- aGGaGGUCGCGG-ACCGCGCGGaagac -5' |
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| 21312 | 5' | -61.5 | NC_004778.3 | + | 111525 | 0.78 | 0.119339 |
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Target: 5'- aCCUCgCAGCGCgUgacGGUGCGCCUUUUGg -3' miRNA: 3'- aGGAG-GUCGCGgA---CCGCGCGGAAGAC- -5' |
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| 21312 | 5' | -61.5 | NC_004778.3 | + | 45586 | 1.07 | 0.000922 |
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Target: 5'- cUCCUCCAGCGCCUGGCGCGCCUUCUGc -3' miRNA: 3'- -AGGAGGUCGCGGACCGCGCGGAAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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