Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21315 | 3' | -58 | NC_004778.3 | + | 44322 | 0.67 | 0.693947 |
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Target: 5'- cGCC-AGCGACgagcGGCGCCGcgAGGa- -3' miRNA: 3'- aCGGaUCGCUGa---CCGUGGCugUCCau -5' |
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| 21315 | 3' | -58 | NC_004778.3 | + | 81736 | 0.67 | 0.683716 |
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Target: 5'- cGCCU-GCGAUauccGGCG-CGGCGGGUGg -3' miRNA: 3'- aCGGAuCGCUGa---CCGUgGCUGUCCAU- -5' |
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| 21315 | 3' | -58 | NC_004778.3 | + | 94743 | 0.67 | 0.673442 |
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Target: 5'- cGUCUGGCuGCUGGCcgagACCGAaaCGGGg- -3' miRNA: 3'- aCGGAUCGcUGACCG----UGGCU--GUCCau -5' |
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| 21315 | 3' | -58 | NC_004778.3 | + | 48975 | 0.68 | 0.660035 |
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Target: 5'- cUGCC-AGCGcacguacaucacgaACUGGC-CCGACcGGUAc -3' miRNA: 3'- -ACGGaUCGC--------------UGACCGuGGCUGuCCAU- -5' |
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| 21315 | 3' | -58 | NC_004778.3 | + | 110114 | 0.69 | 0.570207 |
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Target: 5'- cGCCgAGCG-CUugGGCACCGGCcGGUc -3' miRNA: 3'- aCGGaUCGCuGA--CCGUGGCUGuCCAu -5' |
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| 21315 | 3' | -58 | NC_004778.3 | + | 53153 | 0.69 | 0.570207 |
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Target: 5'- uUGCUgUGGCGGC-GGUugCGGCGGGg- -3' miRNA: 3'- -ACGG-AUCGCUGaCCGugGCUGUCCau -5' |
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| 21315 | 3' | -58 | NC_004778.3 | + | 33756 | 0.71 | 0.480755 |
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Target: 5'- uUG-CUGGCGcggcACUGGCAUCGAguGGUGc -3' miRNA: 3'- -ACgGAUCGC----UGACCGUGGCUguCCAU- -5' |
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| 21315 | 3' | -58 | NC_004778.3 | + | 5055 | 0.71 | 0.471218 |
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Target: 5'- cGCCgcGCGACUGGCGgCGu--GGUAu -3' miRNA: 3'- aCGGauCGCUGACCGUgGCuguCCAU- -5' |
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| 21315 | 3' | -58 | NC_004778.3 | + | 49581 | 1.07 | 0.001819 |
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Target: 5'- uUGCCUAGCGACUGGCACCGACAGGUAg -3' miRNA: 3'- -ACGGAUCGCUGACCGUGGCUGUCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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