Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21316 | 5' | -55.1 | NC_004778.3 | + | 28893 | 0.66 | 0.88221 |
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Target: 5'- -uGUUuGCGCgUGCGCGAUGAaCGCGUg -3' miRNA: 3'- guCAAcUGCG-ACGCGUUGCUcGCGUA- -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 8780 | 0.66 | 0.885091 |
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Target: 5'- aCAuUUGGCGCaGCagcuucagcggcgagGCGGCGGGCGCGc -3' miRNA: 3'- -GUcAACUGCGaCG---------------CGUUGCUCGCGUa -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 84721 | 0.66 | 0.889341 |
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Target: 5'- cCAGuUUGGCGaUGCGUAACGAGUucuGCu- -3' miRNA: 3'- -GUC-AACUGCgACGCGUUGCUCG---CGua -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 32823 | 0.66 | 0.889341 |
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Target: 5'- gCAGcUGcCGCgGCGCGugGuGCGUAa -3' miRNA: 3'- -GUCaACuGCGaCGCGUugCuCGCGUa -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 61788 | 0.66 | 0.889341 |
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Target: 5'- -cGUUGGCGC-GCGCuggcuGCGcGGCGCc- -3' miRNA: 3'- guCAACUGCGaCGCGu----UGC-UCGCGua -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 88075 | 0.66 | 0.889341 |
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Target: 5'- aCAG-UGGCuGUUGCGCAuGCGAuGUGCGa -3' miRNA: 3'- -GUCaACUG-CGACGCGU-UGCU-CGCGUa -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 106454 | 0.66 | 0.889341 |
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Target: 5'- gGGcUGGgGCUGCgGCGggcACGGGCGCu- -3' miRNA: 3'- gUCaACUgCGACG-CGU---UGCUCGCGua -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 111561 | 0.66 | 0.896228 |
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Target: 5'- -cGUUGA-GCUG-GCGAcCGGGCGCGc -3' miRNA: 3'- guCAACUgCGACgCGUU-GCUCGCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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