Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21316 | 5' | -55.1 | NC_004778.3 | + | 114916 | 0.69 | 0.720334 |
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Target: 5'- uGGUgGACGCUaaaGCGCAagccgGCGuGCGCAc -3' miRNA: 3'- gUCAaCUGCGA---CGCGU-----UGCuCGCGUa -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 116823 | 0.66 | 0.88221 |
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Target: 5'- gCGGUUGACagacagaauGCugagaaUGCGCAGuuUGGGCGCGUa -3' miRNA: 3'- -GUCAACUG---------CG------ACGCGUU--GCUCGCGUA- -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 118203 | 0.67 | 0.85137 |
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Target: 5'- --cUUGGCGCUcauCGACGAGCGCAg -3' miRNA: 3'- gucAACUGCGAcgcGUUGCUCGCGUa -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 118781 | 0.68 | 0.79891 |
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Target: 5'- ----gGGCGCgGCGCGGCcGGCGCGc -3' miRNA: 3'- gucaaCUGCGaCGCGUUGcUCGCGUa -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 121130 | 0.75 | 0.406759 |
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Target: 5'- aAGgcGGCGCcgGgGCAGCGGGCGCGg -3' miRNA: 3'- gUCaaCUGCGa-CgCGUUGCUCGCGUa -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 122551 | 0.66 | 0.859416 |
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Target: 5'- aGGUcGGCGCUGaCGCAcGCGA-CGCAc -3' miRNA: 3'- gUCAaCUGCGAC-GCGU-UGCUcGCGUa -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 128650 | 0.67 | 0.82247 |
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Target: 5'- aAGUUGGCGCUGCaGCAucaaucgaauuguCGAGCaacggcggagGCAUg -3' miRNA: 3'- gUCAACUGCGACG-CGUu------------GCUCG----------CGUA- -5' |
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| 21316 | 5' | -55.1 | NC_004778.3 | + | 128731 | 0.67 | 0.831207 |
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Target: 5'- aAGUUGGCGCUGCaGCAucaaucgaauuguCGAGCaacgGCGg -3' miRNA: 3'- gUCAACUGCGACG-CGUu------------GCUCG----CGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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