Results 41 - 48 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21316 | 5' | -55.1 | NC_004778.3 | + | 10720 | 0.66 | 0.88221 |
Target: 5'- uGGUUGGaccgcuguuCGCUGCGCGcuccGCGGGCuGCc- -3' miRNA: 3'- gUCAACU---------GCGACGCGU----UGCUCG-CGua -5' |
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21316 | 5' | -55.1 | NC_004778.3 | + | 8780 | 0.66 | 0.885091 |
Target: 5'- aCAuUUGGCGCaGCagcuucagcggcgagGCGGCGGGCGCGc -3' miRNA: 3'- -GUcAACUGCGaCG---------------CGUUGCUCGCGUa -5' |
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21316 | 5' | -55.1 | NC_004778.3 | + | 114352 | 0.66 | 0.859416 |
Target: 5'- gCGGuUUGACcCUGCGCGccGCGGGCGa-- -3' miRNA: 3'- -GUC-AACUGcGACGCGU--UGCUCGCgua -5' |
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21316 | 5' | -55.1 | NC_004778.3 | + | 91357 | 0.67 | 0.834648 |
Target: 5'- ----aGACGCUGUuCGACGAcaGCGCAa -3' miRNA: 3'- gucaaCUGCGACGcGUUGCU--CGCGUa -5' |
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21316 | 5' | -55.1 | NC_004778.3 | + | 53669 | 0.67 | 0.817138 |
Target: 5'- -cGUUGagGCGCUGCcCAAUGcGGCGCAa -3' miRNA: 3'- guCAAC--UGCGACGcGUUGC-UCGCGUa -5' |
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21316 | 5' | -55.1 | NC_004778.3 | + | 52291 | 0.67 | 0.817138 |
Target: 5'- uCGGUUGGCGUUGUGCAAaaacuuguccAGCGCc- -3' miRNA: 3'- -GUCAACUGCGACGCGUUgc--------UCGCGua -5' |
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21316 | 5' | -55.1 | NC_004778.3 | + | 118781 | 0.68 | 0.79891 |
Target: 5'- ----gGGCGCgGCGCGGCcGGCGCGc -3' miRNA: 3'- gucaaCUGCGaCGCGUUGcUCGCGUa -5' |
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21316 | 5' | -55.1 | NC_004778.3 | + | 57996 | 1.06 | 0.003912 |
Target: 5'- uCAGUUGACGCUGCGCAACGAGCGCAUg -3' miRNA: 3'- -GUCAACUGCGACGCGUUGCUCGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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