Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21318 | 3' | -51.1 | NC_004778.3 | + | 52325 | 0.67 | 0.972203 |
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Target: 5'- gCUGCCUUuucgccaAACGAGCUGCcguugcGCUUGa -3' miRNA: 3'- -GAUGGGAuac----UUGCUCGACGa-----CGAAC- -5' |
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| 21318 | 3' | -51.1 | NC_004778.3 | + | 80677 | 0.67 | 0.971908 |
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Target: 5'- -gACCCgcgAaucuacgacagccUGAGCGAGCUGCgcgaCUUGg -3' miRNA: 3'- gaUGGGa--U-------------ACUUGCUCGACGac--GAAC- -5' |
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| 21318 | 3' | -51.1 | NC_004778.3 | + | 90411 | 0.67 | 0.967213 |
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Target: 5'- -gACC--AUGAACGGGCUGUguuuuggaagcguaaUGCUUGu -3' miRNA: 3'- gaUGGgaUACUUGCUCGACG---------------ACGAAC- -5' |
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| 21318 | 3' | -51.1 | NC_004778.3 | + | 42519 | 0.67 | 0.962354 |
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Target: 5'- gUGCCCgac-AACGGGCUGCUcaaCUUGg -3' miRNA: 3'- gAUGGGauacUUGCUCGACGAc--GAAC- -5' |
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| 21318 | 3' | -51.1 | NC_004778.3 | + | 40736 | 0.68 | 0.954577 |
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Target: 5'- aUGCauauuUGAugGAGUUGCUGCUg- -3' miRNA: 3'- gAUGggau-ACUugCUCGACGACGAac -5' |
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| 21318 | 3' | -51.1 | NC_004778.3 | + | 117331 | 0.68 | 0.950309 |
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Target: 5'- -aGCCCauuugcgggGUGGcuccagGCGAGCUGCUGUUg- -3' miRNA: 3'- gaUGGGa--------UACU------UGCUCGACGACGAac -5' |
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| 21318 | 3' | -51.1 | NC_004778.3 | + | 115064 | 0.7 | 0.899971 |
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Target: 5'- cCUGCCCgucgAUGA--GGGCUGCUGUc-- -3' miRNA: 3'- -GAUGGGa---UACUugCUCGACGACGaac -5' |
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| 21318 | 3' | -51.1 | NC_004778.3 | + | 50172 | 0.71 | 0.854709 |
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Target: 5'- -cACCUuUAUGAACGAGCUGCUa---- -3' miRNA: 3'- gaUGGG-AUACUUGCUCGACGAcgaac -5' |
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| 21318 | 3' | -51.1 | NC_004778.3 | + | 95197 | 0.77 | 0.549114 |
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Target: 5'- aCUGCCCggcUGccGACGGGCUGCUGCc-- -3' miRNA: 3'- -GAUGGGau-AC--UUGCUCGACGACGaac -5' |
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| 21318 | 3' | -51.1 | NC_004778.3 | + | 64307 | 1.09 | 0.005609 |
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Target: 5'- gCUACCCUAUGAACGAGCUGCUGCUUGa -3' miRNA: 3'- -GAUGGGAUACUUGCUCGACGACGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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