Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21330 | 3' | -56.6 | NC_004778.3 | + | 45282 | 0.66 | 0.79327 |
Target: 5'- cGCGCGGCCGGGucGcUCUccaCAGCGGg -3' miRNA: 3'- uUGCGUCGGCCUcuC-AGAa--GUCGCUa -5' |
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21330 | 3' | -56.6 | NC_004778.3 | + | 57379 | 0.67 | 0.76465 |
Target: 5'- -uCGCGGCCGGucGAGUCcaCAGCu-- -3' miRNA: 3'- uuGCGUCGGCCu-CUCAGaaGUCGcua -5' |
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21330 | 3' | -56.6 | NC_004778.3 | + | 14322 | 0.71 | 0.497199 |
Target: 5'- -cUGguGCCGGcgaaauaacguGGGUCUUCGGCGAUg -3' miRNA: 3'- uuGCguCGGCCu----------CUCAGAAGUCGCUA- -5' |
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21330 | 3' | -56.6 | NC_004778.3 | + | 80372 | 1.03 | 0.004214 |
Target: 5'- gAACGCAGCCGGAGAGUCUUCAGCGAUu -3' miRNA: 3'- -UUGCGUCGGCCUCUCAGAAGUCGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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