Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21333 | 5' | -58.1 | NC_004778.3 | + | 8892 | 0.69 | 0.57904 |
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Target: 5'- -gGUGGCGC-GC-CACgGCGCC-GGCGg -3' miRNA: 3'- aaCAUCGCGaCGaGUG-UGCGGuCCGC- -5' |
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| 21333 | 5' | -58.1 | NC_004778.3 | + | 88130 | 0.68 | 0.609051 |
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Target: 5'- ---gGGCGCUGUuaaaaaaUUACGCGCCGccuGGCGu -3' miRNA: 3'- aacaUCGCGACG-------AGUGUGCGGU---CCGC- -5' |
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| 21333 | 5' | -58.1 | NC_004778.3 | + | 73191 | 0.67 | 0.651688 |
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Target: 5'- ---gAGuCGCUGCUCugauuCAgGCUGGGCGa -3' miRNA: 3'- aacaUC-GCGACGAGu----GUgCGGUCCGC- -5' |
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| 21333 | 5' | -58.1 | NC_004778.3 | + | 93164 | 0.66 | 0.75326 |
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Target: 5'- gUUGgGGCuGCUGCgcguUCACGCGgCC-GGCGg -3' miRNA: 3'- -AACaUCG-CGACG----AGUGUGC-GGuCCGC- -5' |
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| 21333 | 5' | -58.1 | NC_004778.3 | + | 33239 | 0.72 | 0.396807 |
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Target: 5'- gUGUAGCGCggcagcgggccGCUgCACcguuGCGCCAGGCu -3' miRNA: 3'- aACAUCGCGa----------CGA-GUG----UGCGGUCCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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