Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21334 | 3' | -46.4 | NC_004778.3 | + | 118728 | 0.66 | 0.999865 |
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Target: 5'- cUGUGUGCGcuuGGCCUcg----GCGCGGc -3' miRNA: 3'- aGCAUAUGU---CCGGAaaauaaCGCGUC- -5' |
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| 21334 | 3' | -46.4 | NC_004778.3 | + | 34432 | 0.66 | 0.999865 |
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Target: 5'- cUCGUAgGCGGGCUcaaauaUGCGCGc -3' miRNA: 3'- -AGCAUaUGUCCGGaaaauaACGCGUc -5' |
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| 21334 | 3' | -46.4 | NC_004778.3 | + | 11757 | 0.66 | 0.999772 |
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Target: 5'- gCGgcgACAGGCgga--GUUGCGCGGa -3' miRNA: 3'- aGCauaUGUCCGgaaaaUAACGCGUC- -5' |
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| 21334 | 3' | -46.4 | NC_004778.3 | + | 75488 | 0.67 | 0.999492 |
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Target: 5'- aCGUugccgccGCuuuGGCCUUcaccgacagugaccUUGUUGCGCAGu -3' miRNA: 3'- aGCAua-----UGu--CCGGAA--------------AAUAACGCGUC- -5' |
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| 21334 | 3' | -46.4 | NC_004778.3 | + | 33241 | 0.68 | 0.998863 |
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Target: 5'- aCGUGUagcgcggcaGCGGGCCgcugcaccGUUGCGcCAGg -3' miRNA: 3'- aGCAUA---------UGUCCGGaaaa----UAACGC-GUC- -5' |
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| 21334 | 3' | -46.4 | NC_004778.3 | + | 114732 | 0.68 | 0.998863 |
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Target: 5'- cCGUGUGCAaaacGGCUcgcaAUUGCGCAu -3' miRNA: 3'- aGCAUAUGU----CCGGaaaaUAACGCGUc -5' |
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| 21334 | 3' | -46.4 | NC_004778.3 | + | 3 | 0.68 | 0.99791 |
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Target: 5'- -aGUAUGCGGGCCcggugaacagagGCGCAu -3' miRNA: 3'- agCAUAUGUCCGGaaaauaa-----CGCGUc -5' |
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| 21334 | 3' | -46.4 | NC_004778.3 | + | 128174 | 0.68 | 0.997532 |
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Target: 5'- gCGUGUGC-GGCUgcagUUGUUGUGgAGg -3' miRNA: 3'- aGCAUAUGuCCGGaa--AAUAACGCgUC- -5' |
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| 21334 | 3' | -46.4 | NC_004778.3 | + | 38376 | 0.74 | 0.944823 |
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Target: 5'- cUGUGUACuGGCCg-----UGCGCAGc -3' miRNA: 3'- aGCAUAUGuCCGGaaaauaACGCGUC- -5' |
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| 21334 | 3' | -46.4 | NC_004778.3 | + | 91272 | 1.12 | 0.013745 |
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Target: 5'- uUCGUAUACAGGCCUUUUAUUGCGCAGa -3' miRNA: 3'- -AGCAUAUGUCCGGAAAAUAACGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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