Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 21335 | 5' | -55.8 | NC_004778.3 | + | 76289 | 0.74 | 0.4322 |
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Target: 5'- cGCGCGCCGUgaACGGcuCGCAUUugGCAAGc -3' miRNA: 3'- aCGCGCGGCG--UGCCu-GUGUAA--CGUUU- -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 95849 | 0.74 | 0.413991 |
|
Target: 5'- aUGCGCGCCguaaGCACGGGCgACAUgaccaGCGGu -3' miRNA: 3'- -ACGCGCGG----CGUGCCUG-UGUAa----CGUUu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 49841 | 0.76 | 0.322786 |
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Target: 5'- cUGgGCcCCGCACGGGCGCGcUUGCGGg -3' miRNA: 3'- -ACgCGcGGCGUGCCUGUGU-AACGUUu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 89588 | 0.77 | 0.279727 |
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Target: 5'- aGuCGCGCCGCACGcGCGCAUUGUu-- -3' miRNA: 3'- aC-GCGCGGCGUGCcUGUGUAACGuuu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 10257 | 0.8 | 0.187246 |
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Target: 5'- aGCGCGCCgauugcGCGCGGgaccggcgGCGCAUUGCAAAg -3' miRNA: 3'- aCGCGCGG------CGUGCC--------UGUGUAACGUUU- -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 94853 | 1.06 | 0.003229 |
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Target: 5'- uUGCGCGCCGCACGGACACAUUGCAAAc -3' miRNA: 3'- -ACGCGCGGCGUGCCUGUGUAACGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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