Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21335 | 5' | -55.8 | NC_004778.3 | + | 4607 | 0.66 | 0.848746 |
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Target: 5'- cGCGUgccagGCCGCGCGauuccggacgaaGACACugUGCAAAa -3' miRNA: 3'- aCGCG-----CGGCGUGC------------CUGUGuaACGUUU- -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 32826 | 0.66 | 0.848746 |
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Target: 5'- cGCGCaGCUGcCGCGG-CGCGUggUGCGu- -3' miRNA: 3'- aCGCG-CGGC-GUGCCuGUGUA--ACGUuu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 11766 | 0.66 | 0.856838 |
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Target: 5'- gGCGgaGuuGCGCGGACcCGUUaaaGCAAAa -3' miRNA: 3'- aCGCg-CggCGUGCCUGuGUAA---CGUUU- -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 65763 | 0.66 | 0.856838 |
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Target: 5'- aGCGaGCCGgGCGGugACg--GCGAu -3' miRNA: 3'- aCGCgCGGCgUGCCugUGuaaCGUUu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 37859 | 0.66 | 0.864713 |
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Target: 5'- cGaCGCGCaCGCGCaGACu--UUGCAAAg -3' miRNA: 3'- aC-GCGCG-GCGUGcCUGuguAACGUUU- -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 47203 | 0.66 | 0.864713 |
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Target: 5'- gGCGCGCUGCagaaaaucaaGCuGACGCAcgGCGu- -3' miRNA: 3'- aCGCGCGGCG----------UGcCUGUGUaaCGUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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