Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21335 | 5' | -55.8 | NC_004778.3 | + | 118868 | 0.68 | 0.767514 |
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Target: 5'- cGCGCGCaCGCAacugaGGACAaa-UGCGu- -3' miRNA: 3'- aCGCGCG-GCGUg----CCUGUguaACGUuu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 121701 | 0.68 | 0.727667 |
|
Target: 5'- cGUGCaggcCCGCGuCGGGCACGUUGUc-- -3' miRNA: 3'- aCGCGc---GGCGU-GCCUGUGUAACGuuu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 121864 | 0.67 | 0.823248 |
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Target: 5'- gGCGCGCCGacaACGugcccGACGCGggccUGCAc- -3' miRNA: 3'- aCGCGCGGCg--UGC-----CUGUGUa---ACGUuu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 124220 | 0.69 | 0.686416 |
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Target: 5'- aGCGCGCCgugcaucuGCACGaGCAC-UUGCAc- -3' miRNA: 3'- aCGCGCGG--------CGUGCcUGUGuAACGUuu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 124566 | 0.67 | 0.77718 |
|
Target: 5'- cGCGCGCCgagucacguagGCGCGcGCGCAacuuuuUUGCAGu -3' miRNA: 3'- aCGCGCGG-----------CGUGCcUGUGU------AACGUUu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 129295 | 0.67 | 0.805312 |
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Target: 5'- aUGCugGCGCUGUacgccGCGGACGCGccGCAGu -3' miRNA: 3'- -ACG--CGCGGCG-----UGCCUGUGUaaCGUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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