Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21335 | 5' | -55.8 | NC_004778.3 | + | 115298 | 0.68 | 0.746809 |
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Target: 5'- aUGCGCcgaaaaaauugagGCgGC-CGGccGCACAUUGCAAAu -3' miRNA: 3'- -ACGCG-------------CGgCGuGCC--UGUGUAACGUUU- -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 48080 | 0.68 | 0.761651 |
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Target: 5'- gGUGUGCgGCGCGGACauuuaccacaauacgGCGguacUGCAGAa -3' miRNA: 3'- aCGCGCGgCGUGCCUG---------------UGUa---ACGUUU- -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 106461 | 0.68 | 0.767514 |
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Target: 5'- aGCGCGCUGCGCGcGCAaaacgcGCAAc -3' miRNA: 3'- aCGCGCGGCGUGCcUGUguaa--CGUUu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 57950 | 0.68 | 0.767514 |
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Target: 5'- cGCGCGCCuGCGCuGcGCugGcUGCAAAg -3' miRNA: 3'- aCGCGCGG-CGUGcC-UGugUaACGUUU- -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 118868 | 0.68 | 0.767514 |
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Target: 5'- cGCGCGCaCGCAacugaGGACAaa-UGCGu- -3' miRNA: 3'- aCGCGCG-GCGUg----CCUGUguaACGUuu -5' |
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| 21335 | 5' | -55.8 | NC_004778.3 | + | 11766 | 0.66 | 0.856838 |
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Target: 5'- gGCGgaGuuGCGCGGACcCGUUaaaGCAAAa -3' miRNA: 3'- aCGCg-CggCGUGCCUGuGUAA---CGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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