Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21336 | 5' | -54.5 | NC_004778.3 | + | 7171 | 0.69 | 0.778351 |
|
Target: 5'- aGGCuAUGACCGCGAcgauauGCGCGACAaGAUc -3' miRNA: 3'- -UUGuUGCUGGCGCU------CGCGUUGUgCUA- -5' |
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| 21336 | 5' | -54.5 | NC_004778.3 | + | 5129 | 0.67 | 0.873585 |
|
Target: 5'- aAACAACGcGCCGUGgcaaugucgcGGCGCAcGCAUGAc -3' miRNA: 3'- -UUGUUGC-UGGCGC----------UCGCGU-UGUGCUa -5' |
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| 21336 | 5' | -54.5 | NC_004778.3 | + | 2397 | 0.69 | 0.777389 |
|
Target: 5'- aAGCGGCGuacguCCGCGGGCGUGACGuuucuguCGAUc -3' miRNA: 3'- -UUGUUGCu----GGCGCUCGCGUUGU-------GCUA- -5' |
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| 21336 | 5' | -54.5 | NC_004778.3 | + | 2178 | 0.68 | 0.82448 |
|
Target: 5'- cGACuACGACCGaGAGCaGCuucGACGCGAUu -3' miRNA: 3'- -UUGuUGCUGGCgCUCG-CG---UUGUGCUA- -5' |
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| 21336 | 5' | -54.5 | NC_004778.3 | + | 1658 | 0.68 | 0.806527 |
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Target: 5'- cGGCAGCGccCCGCGGG-GCAACAUGu- -3' miRNA: 3'- -UUGUUGCu-GGCGCUCgCGUUGUGCua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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