Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21337 | 3' | -52.1 | NC_004778.3 | + | 121701 | 0.68 | 0.9378 |
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Target: 5'- gGcCGGCGUGgg-CGUGUUGcuggccGGCGGCg -3' miRNA: 3'- gC-GUCGCACgaaGCACAACa-----UCGUCG- -5' |
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| 21337 | 3' | -52.1 | NC_004778.3 | + | 41252 | 0.68 | 0.9378 |
|
Target: 5'- aGCuGCGUGCUgcacaaaugcgUCGUGguggUGUugGGCAccGCg -3' miRNA: 3'- gCGuCGCACGA-----------AGCACa---ACA--UCGU--CG- -5' |
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| 21337 | 3' | -52.1 | NC_004778.3 | + | 104005 | 0.67 | 0.947315 |
|
Target: 5'- cCGUGGCGUGUgggugUUCGgcaaggaGUUGgugaAGCGGCa -3' miRNA: 3'- -GCGUCGCACG-----AAGCa------CAACa---UCGUCG- -5' |
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| 21337 | 3' | -52.1 | NC_004778.3 | + | 38256 | 0.67 | 0.949095 |
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Target: 5'- gGaCGGCGUGCgcgcuagCGUGcUGUugcgcgucgcgccgcAGCGGCa -3' miRNA: 3'- gC-GUCGCACGaa-----GCACaACA---------------UCGUCG- -5' |
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| 21337 | 3' | -52.1 | NC_004778.3 | + | 53161 | 0.66 | 0.963332 |
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Target: 5'- gGCGGCGguUGCUguggCGgcgGUUGcGGCGGg -3' miRNA: 3'- gCGUCGC--ACGAa---GCa--CAACaUCGUCg -5' |
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| 21337 | 3' | -52.1 | NC_004778.3 | + | 60337 | 0.66 | 0.972857 |
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Target: 5'- aGCGGCGUGCcgUUGcaaaagGUGGCGcGCu -3' miRNA: 3'- gCGUCGCACGa-AGCacaa--CAUCGU-CG- -5' |
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| 21337 | 3' | -52.1 | NC_004778.3 | + | 34594 | 0.76 | 0.559829 |
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Target: 5'- gCGCAGCGUGCc-CGUGUac-AGCAGUa -3' miRNA: 3'- -GCGUCGCACGaaGCACAacaUCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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