Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21376 | 5' | -56.5 | NC_004812.1 | + | 7942 | 0.69 | 0.757029 |
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Target: 5'- cGCGGccgGGGGGcgcGUCCG-GGGGGGGCc -3' miRNA: 3'- -UGUCa--UCCCCu--CAGGCaCUCUCUCGc -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 7491 | 0.66 | 0.908617 |
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Target: 5'- -gGGaUGGGGGGGggaCgGUGGGccGGAGCGa -3' miRNA: 3'- ugUC-AUCCCCUCa--GgCACUC--UCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 6652 | 0.74 | 0.479132 |
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Target: 5'- -gGGUGGGGGAGaUgG-GGGAGAGCGg -3' miRNA: 3'- ugUCAUCCCCUCaGgCaCUCUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 5956 | 0.74 | 0.469783 |
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Target: 5'- gGCAGggcccGGGGAGgCCGcGGGGGGGCGa -3' miRNA: 3'- -UGUCau---CCCCUCaGGCaCUCUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 5709 | 0.66 | 0.896062 |
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Target: 5'- cACGGgggGGcGGGGGUCUGccgcggGAGgAGGGCGc -3' miRNA: 3'- -UGUCa--UC-CCCUCAGGCa-----CUC-UCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 5337 | 0.69 | 0.775811 |
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Target: 5'- cACGGaggcgGGGGGAGgcCCGgGAGAG-GCGg -3' miRNA: 3'- -UGUCa----UCCCCUCa-GGCaCUCUCuCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 5105 | 0.7 | 0.688331 |
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Target: 5'- -aGGcGGGGGAGUCUGggguccGGGGGGCGg -3' miRNA: 3'- ugUCaUCCCCUCAGGCac----UCUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 4778 | 0.69 | 0.72806 |
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Target: 5'- cGCGGUGGGGGAGggggCgG-GGcGAGGGCu -3' miRNA: 3'- -UGUCAUCCCCUCa---GgCaCU-CUCUCGc -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 33 | 0.68 | 0.811783 |
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Target: 5'- uCGGggGGcGGGGGUUCGUucGGGGGGGCGc -3' miRNA: 3'- uGUCa-UC-CCCUCAGGCA--CUCUCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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