Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21376 | 5' | -56.5 | NC_004812.1 | + | 155062 | 0.67 | 0.828837 |
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Target: 5'- cGCGcGUAGGGGGGaa-GgggGGGGGGGCGg -3' miRNA: 3'- -UGU-CAUCCCCUCaggCa--CUCUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 27793 | 0.67 | 0.845183 |
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Target: 5'- cCGGgggagAGGGcGGGgCCGggGGGGGAGCGg -3' miRNA: 3'- uGUCa----UCCC-CUCaGGCa-CUCUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 80690 | 0.67 | 0.860766 |
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Target: 5'- cCAGgucccUGGGGGGGUCCGgcccgGGGaAGGGaCGa -3' miRNA: 3'- uGUC-----AUCCCCUCAGGCa----CUC-UCUC-GC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 77937 | 0.67 | 0.860766 |
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Target: 5'- aGCGGUucGGGGuGGGUCgggGUGGcGGGGGCGa -3' miRNA: 3'- -UGUCA--UCCC-CUCAGg--CACU-CUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 35553 | 0.66 | 0.882597 |
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Target: 5'- cGCGGgcGGGGc--CCGgauagaggGAGGGGGCGg -3' miRNA: 3'- -UGUCauCCCCucaGGCa-------CUCUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 148483 | 0.66 | 0.88673 |
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Target: 5'- cGCGGgggAGGGGAGggcgaggggugcgCgGgggagGGGAGGGCGa -3' miRNA: 3'- -UGUCa--UCCCCUCa------------GgCa----CUCUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 148535 | 0.66 | 0.88673 |
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Target: 5'- cGCGGgggAGGGGAGggcgaggggugcgCgGgggagGGGAGGGCGa -3' miRNA: 3'- -UGUCa--UCCCCUCa------------GgCa----CUCUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 148587 | 0.66 | 0.88673 |
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Target: 5'- cGCGGgggAGGGGAGggcgaggggugcgCgGgggagGGGAGGGCGa -3' miRNA: 3'- -UGUCa--UCCCCUCa------------GgCa----CUCUCUCGC- -5' |
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| 21376 | 5' | -56.5 | NC_004812.1 | + | 122547 | 0.66 | 0.908617 |
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Target: 5'- -gGGgcGGGGGG-CgCGgGGGGGGGCGg -3' miRNA: 3'- ugUCauCCCCUCaG-GCaCUCUCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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