miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2138 3' -53.3 NC_001362.1 + 6214 0.66 0.251307
Target:  5'- aUGAguUACcugGUCCAgcuuaagUCUgUUCCAGGCa -3'
miRNA:   3'- aACU--AUGa--CAGGUa------AGGgAGGGUCCG- -5'
2138 3' -53.3 NC_001362.1 + 700 0.66 0.236311
Target:  5'- gUUGuggGCUGUCCGUUCgacauCCUUCCAguGGUc -3'
miRNA:   3'- -AACua-UGACAGGUAAG-----GGAGGGU--CCG- -5'
2138 3' -53.3 NC_001362.1 + 5966 0.7 0.132656
Target:  5'- cUGAUAUUGcCCAUacugucucccgaUCCCcauuggucacuUCCCAGGUa -3'
miRNA:   3'- aACUAUGACaGGUA------------AGGG-----------AGGGUCCG- -5'
2138 3' -53.3 NC_001362.1 + 1304 1.11 0.000062
Target:  5'- aUUGAUACUGUCCAUUCCCUCCCAGGCg -3'
miRNA:   3'- -AACUAUGACAGGUAAGGGAGGGUCCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.