Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21381 | 5' | -56.7 | NC_004812.1 | + | 49416 | 0.66 | 0.888952 |
Target: 5'- -cUCUCCCcGCCCGaccgccacgcuccGCUCGCCcggCGc -3' miRNA: 3'- caAGAGGGaCGGGCa------------CGAGUGGaa-GC- -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 74708 | 0.66 | 0.863163 |
Target: 5'- cGUUCgCCCUGCggGUGCUgGCCUg-- -3' miRNA: 3'- -CAAGaGGGACGggCACGAgUGGAagc -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 22092 | 0.67 | 0.847662 |
Target: 5'- --gCUCCacaUGCCCG-GCUCcaGCCggcgUCGg -3' miRNA: 3'- caaGAGGg--ACGGGCaCGAG--UGGa---AGC- -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 137859 | 0.67 | 0.831384 |
Target: 5'- --cCUCgUCUGCCgGgccCUCACCUUCGa -3' miRNA: 3'- caaGAG-GGACGGgCac-GAGUGGAAGC- -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 40910 | 0.67 | 0.822971 |
Target: 5'- -gUC-CCCaUGCCCuggGCUCGCCU-CGg -3' miRNA: 3'- caAGaGGG-ACGGGca-CGAGUGGAaGC- -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 110299 | 0.67 | 0.814386 |
Target: 5'- -gUgUgCCUGCCCGUGCcCGCCccguacgUCGu -3' miRNA: 3'- caAgAgGGACGGGCACGaGUGGa------AGC- -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 103720 | 0.68 | 0.79673 |
Target: 5'- -cUCgagCCCUGCaCCGUcggucaccggcGCuacuUCACCUUCGg -3' miRNA: 3'- caAGa--GGGACG-GGCA-----------CG----AGUGGAAGC- -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 33557 | 0.69 | 0.7405 |
Target: 5'- -----aCCgGCCCGUGCUCACCa--- -3' miRNA: 3'- caagagGGaCGGGCACGAGUGGaagc -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 113310 | 0.69 | 0.730747 |
Target: 5'- -gUUUCCCaacgGCaCCGUGaUUCACCUUCu -3' miRNA: 3'- caAGAGGGa---CG-GGCAC-GAGUGGAAGc -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 153293 | 0.71 | 0.640228 |
Target: 5'- cUUC-CCCgagGCCUGgcgcgccgcGCUCGCCUUCGa -3' miRNA: 3'- cAAGaGGGa--CGGGCa--------CGAGUGGAAGC- -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 27785 | 0.71 | 0.640228 |
Target: 5'- cUUC-CCCgagGCCUGgcgcgccgcGCUCGCCUUCGa -3' miRNA: 3'- cAAGaGGGa--CGGGCa--------CGAGUGGAAGC- -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 98471 | 0.73 | 0.509757 |
Target: 5'- -----gCCUGCCCGcUGCUCAUCUUUGa -3' miRNA: 3'- caagagGGACGGGC-ACGAGUGGAAGC- -5' |
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21381 | 5' | -56.7 | NC_004812.1 | + | 143073 | 1.08 | 0.002935 |
Target: 5'- uGUUCUCCCUGCCCGUGCUCACCUUCGu -3' miRNA: 3'- -CAAGAGGGACGGGCACGAGUGGAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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