Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21384 | 3' | -51.9 | NC_004812.1 | + | 153471 | 0.67 | 0.968919 |
Target: 5'- cGCGUGCUGGUGCUCUaCGcg--CCg- -3' miRNA: 3'- -UGCGCGACUACGAGAaGUcuuaGGac -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 28160 | 0.67 | 0.968919 |
Target: 5'- gGCGUGCUccUGCUCUccacgCGGGA-CCUGg -3' miRNA: 3'- -UGCGCGAcuACGAGAa----GUCUUaGGAC- -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 27962 | 0.67 | 0.968919 |
Target: 5'- cGCGUGCUGGUGCUCUaCGcg--CCg- -3' miRNA: 3'- -UGCGCGACUACGAGAaGUcuuaGGac -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 95113 | 0.67 | 0.965689 |
Target: 5'- uACGUGCUGggGUUCgcggUCGGGaccgcgGUCCUc -3' miRNA: 3'- -UGCGCGACuaCGAGa---AGUCU------UAGGAc -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 85428 | 0.68 | 0.958535 |
Target: 5'- cCGCGgUGucgcGCUCUaCGGAAUCCUu -3' miRNA: 3'- uGCGCgACua--CGAGAaGUCUUAGGAc -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 23335 | 0.68 | 0.941307 |
Target: 5'- gACGaaCGC-GAUGCUCUUCGGcgcccUCCUGu -3' miRNA: 3'- -UGC--GCGaCUACGAGAAGUCuu---AGGAC- -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 85212 | 0.68 | 0.941307 |
Target: 5'- cACGagaCGCUGcgGUUCUUUGGGAgCCUGg -3' miRNA: 3'- -UGC---GCGACuaCGAGAAGUCUUaGGAC- -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 75248 | 0.69 | 0.907188 |
Target: 5'- gGCGCGCUGcUGCUUggcgCGGAccagcgcGUCCUu -3' miRNA: 3'- -UGCGCGACuACGAGaa--GUCU-------UAGGAc -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 152763 | 0.71 | 0.847571 |
Target: 5'- gACGCGCUGGUGCUCaugcgccugcgCGGGGaCCUc -3' miRNA: 3'- -UGCGCGACUACGAGaa---------GUCUUaGGAc -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 27255 | 0.71 | 0.847571 |
Target: 5'- gACGCGCUGGUGCUCaugcgccugcgCGGGGaCCUc -3' miRNA: 3'- -UGCGCGACUACGAGaa---------GUCUUaGGAc -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 57243 | 0.74 | 0.675219 |
Target: 5'- -gGCGCUGcgGCUCggCGaGAUCCUGg -3' miRNA: 3'- ugCGCGACuaCGAGaaGUcUUAGGAC- -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 69215 | 0.78 | 0.507496 |
Target: 5'- cACGCGUcGAUGCUCUUCAGGuagaacUCCUc -3' miRNA: 3'- -UGCGCGaCUACGAGAAGUCUu-----AGGAc -5' |
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21384 | 3' | -51.9 | NC_004812.1 | + | 136054 | 1.08 | 0.007338 |
Target: 5'- cACGCGCUGAUGCUCUUCAGAAUCCUGu -3' miRNA: 3'- -UGCGCGACUACGAGAAGUCUUAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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